. . . . "liquid nitrogen" . "root" . "leaf" . "recalcitrant samples" . "recalcitrant samples" . "pestle" . "mortar" . "extraction buffer" . "Ground samples (leaf, shoot, root, and recalcitrant samples, approximately 0.5-1 g) using 1 ml of the extraction buffer with or without liquid nitrogen in mortar and pestle that are sterilized.CRITICAL STEP The procedure are carried out at room temperature except the centrifugation steps (at 4℃) as well as the time of precipitating of the nucleic acid using the isopropanol (on ice).CRITICAL STEP Severely disrupt the tissues to create the glaze mode of samples." . . . . . . . . . . . . . . "shoot" . . "2021-03-05T11:34:30.681034"^^ . . . . . . . . . . . "RSA" . "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" . "eSiNGrBxHqXmxmmCqrlvmweZZTeW3VTUrw2lTNwq4K2WW1chx/PJ3CRcPlaXad5Wic4dQlwR/7H4c84jKVZo8IUwjwr2r6Xz48Ed9NE+3sVFVVzxf9PySoK8MkasyqBAHRrxOqL3wBaT9oNSczexTwaTkFveF3RvjMPANeaE7+Q=" . . . "2021-03-05T11:34:30.681034"^^ . .