@prefix this: <http://purl.org/np/RAyJ6cwDL8iRxGaDg6PeZ-QMjwzFN99hnEQsC08H2yhaA> .
@prefix sub: <http://purl.org/np/RAyJ6cwDL8iRxGaDg6PeZ-QMjwzFN99hnEQsC08H2yhaA#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/OBI_0000918> rdfs:label "Dulbecco's modified eagle medium" .
  
  <http://purl.obolibrary.org/obo/OBI_1000024> rdfs:label "cell pellet" .
  
  sub:_1 rdfs:label "medium" .
  
  sub:step dc:description "Discard medium and re-suspend the cell pellet in 1 ml of sterile Dulbecco's modified eagle medium.";
    a p-plan:Step;
    bs:bioSampleUsed <http://purl.obolibrary.org/obo/OBI_1000024>;
    bs:reagentUsed <http://purl.obolibrary.org/obo/OBI_0000918>, sub:_1 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-02-14T05:27:16.013336"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:IXNLMG6tEeuzfOcdUB4Nvg, authority:MR2YGm6tEeua50dSALniKg,
      authority:StYJpG6tEeugDptRWDXrUg, authority:X5uYhm6tEeu5occrY5SXuA .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "fsx+RoXFRllp/9pRs7z0vXMsqMpqVpymApEo0biUHK9yj2NYEX6q/S1U0UK8vm/Xkp0P2cia8LPmvamakk/IQki5lnMdl9uLD8OQxDNeFThauegejnSLHR6y6BogNKHShZX6u5TIj6r9h1PkNMsQyqJk96C31Br0dScRSiQ8LVU=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-02-14T05:27:16.013336"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}