<http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY> <http://www.nanopub.org/nschema#hasAssertion> <http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#assertion> <http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#Head> .
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<http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY> <http://www.nanopub.org/nschema#hasPublicationInfo> <http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#pubInfo> <http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#Head> .
<http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://www.nanopub.org/nschema#Nanopublication> <http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#Head> .
<http://purl.org/net/SMARTprotocol#ChloroformIsoamylAlcoholMixture> <http://www.w3.org/2000/01/rdf-schema#label> "chloroform:isoamyl alcohol" <http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#assertion> .
<http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#step> <http://purl.org/dc/elements/1.1/description> "Repeat the above step(5) , then add chloroform:isoamyl alcohol(24:1) and centrifuge at 12,000g for 10 minutes at  4º celcius  ." <http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#assertion> .
<http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#step> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/net/p-plan#Step> <http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#assertion> .
<http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#step> <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed> <http://purl.org/net/SMARTprotocol#ChloroformIsoamylAlcoholMixture> <http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#assertion> .
<http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#assertion> <http://purl.org/pav/createdWith> <https://nanotate.bitsfetch.com> <http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#provenance> .
<http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#assertion> <http://www.w3.org/ns/prov#generatedAtTime> "2021-02-24T16:00:38.023670"^^<http://www.w3.org/2001/XMLSchema#dateTime> <http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#provenance> .
<http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#assertion> <http://www.w3.org/ns/prov#wasAttributedTo> <https://hypothes.is/users/zuluaga.lizzz> <http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#provenance> .
<http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/DiHF3nbhEeujslfdJ8NR8A> <http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#pubInfo> .
<http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/LmSLYHbhEeuWl4_SvauOfg> <http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#pubInfo> .
<http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#sig> <http://purl.org/nanopub/x/hasAlgorithm> "RSA" <http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#pubInfo> .
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<http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#sig> <http://purl.org/nanopub/x/hasSignature> "Z7goZYoe7pkfkJOINxIpjjsbz4iAH7anHc9cypuMZL7OLVv8i1uIN0R9tq1vEpKq6OnLjDmR5LTfS/BsOfH+8s1Cnlo4qTNTOXRzyHFTsFYavMMox4f2ZC290RULUw+Nr2JPEFIRNGUclPvZJ8DgaZbrjnai7/UNUtAaZdxe4KY=" <http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#pubInfo> .
<http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#sig> <http://purl.org/nanopub/x/hasSignatureTarget> <http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY> <http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#pubInfo> .
<http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY> <http://purl.org/nanopub/x/introduces> <http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#step> <http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#pubInfo> .
<http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY> <http://www.w3.org/ns/prov#generatedAtTime> "2021-02-24T16:00:38.023670"^^<http://www.w3.org/2001/XMLSchema#dateTime> <http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#pubInfo> .
<http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY> <http://www.w3.org/ns/prov#wasAttributedTo> <https://hypothes.is/users/zuluaga.lizzz> <http://purl.org/np/RAwdsa4-CdE37sAimf946CXaHzkbi8yhht3RP17dKxsyY#pubInfo> .