@prefix this: <http://purl.org/np/RAwHKkQ8dmPkzhTGOQN0lPMrHLzjbm-73-rXPXrb-vtEY> .
@prefix sub: <http://purl.org/np/RAwHKkQ8dmPkzhTGOQN0lPMrHLzjbm-73-rXPXrb-vtEY#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  sub:_1 rdfs:label "tubes" .
  
  sub:_2 rdfs:label "swing-out bucket" .
  
  sub:step dc:description "Centrifuge the tubes (without any delay) for 30 min at 100 x g in 4 °C in a swing-out bucket. Fixed angle rotors also can be used but would require more caution when separating cells in interphase.";
    a p-plan:Step;
    bs:labEquipmentUsed sub:_1, sub:_2 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-04T15:10:39.161366"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:hK6GzH0jEeueOYfjmWjLNw, authority:msaalH0jEeuljtOq3z64Ug,
      authority:sCWovH0jEeuMj2caW7AzRg .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "WMHnj4yhxOU1dzFkidCo2H6K/LwByfjbpX0OqBZZRqFpEAzzTKZJIOjf7Z73FQcqqjman3rM+9BZwsJyUW5DhQR1lrVxY0mb8QD89hXq4CispCSrDFHq2cgvP2T0nJ5Hy0JCj+dhB+ayX39d95v0R1OCUX91cD1mZjJ5KbopLME=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-04T15:10:39.161366"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}