@prefix this: <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ> .
@prefix sub: <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.bioontology.org/ontology/NCBITAXON/2> rdfs:label "bacteria" .
  
  sub:_1 rdfs:label "pellet " .
  
  sub:_2 rdfs:label "pestle" .
  
  sub:_3 rdfs:label "mortar" .
  
  sub:_4 rdfs:label "liquid N2" .
  
  sub:step dc:description "Snap freeze pellet in liquid N2 and immediately transfer to a mortar. Using a pestle, crush bacteria while repeatedly adding liquid N2 (~20 ml) to it.";
    a p-plan:Step;
    bs:bioSampleUsed <http://purl.bioontology.org/ontology/NCBITAXON/2>, sub:_1;
    bs:labEquipmentUsed sub:_2, sub:_3;
    bs:reagentUsed sub:_4 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-10T11:13:12.232860"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:5T5BtoFSEeuDVNszA62k8g, authority:SsTfhoFTEeugz7M1dQr42w,
      authority:XnU0XoFTEeu03pMhdlRcng, authority:bKJEkIFTEeu4IH819XJVFw, authority:fN60OIFTEeuXvTMD-qL4vw,
      authority:mK8Z8IFTEeuvOSvYxUpUaw .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "N4l+SnKO+1ioBbTttjSPHif+OJ3qBq7H/5m+yE5MQuX7xASSwc+U079Awu1kCXlNTooib3QoI5pdi/kklyNs4yFWOd6Ly8//iRnqNuf+44qRPezHNr8YJGSdX/JMKa3ZYrZ23cMju+zeWDT8+RQJwKpb8TtX27wbCQJbrJrHg+U=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-10T11:13:12.232860"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}