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<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ> <http://www.nanopub.org/nschema#hasProvenance> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#provenance> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#Head> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ> <http://www.nanopub.org/nschema#hasPublicationInfo> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#pubInfo> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#Head> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://www.nanopub.org/nschema#Nanopublication> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#Head> .
<http://purl.bioontology.org/ontology/NCBITAXON/2> <http://www.w3.org/2000/01/rdf-schema#label> "bacteria" <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#assertion> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#_1> <http://www.w3.org/2000/01/rdf-schema#label> "pellet " <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#assertion> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#_2> <http://www.w3.org/2000/01/rdf-schema#label> "pestle" <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#assertion> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#_3> <http://www.w3.org/2000/01/rdf-schema#label> "mortar" <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#assertion> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#_4> <http://www.w3.org/2000/01/rdf-schema#label> "liquid N2" <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#assertion> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#step> <http://purl.org/dc/elements/1.1/description> "Snap freeze pellet in liquid N2 and immediately transfer to a mortar. Using a pestle, crush bacteria while repeatedly adding liquid N2 (~20 ml) to it." <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#assertion> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#step> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/net/p-plan#Step> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#assertion> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#step> <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed> <http://purl.bioontology.org/ontology/NCBITAXON/2> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#assertion> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#step> <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#_1> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#assertion> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#step> <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#_2> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#assertion> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#step> <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#_3> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#assertion> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#step> <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#_4> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#assertion> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#assertion> <http://purl.org/pav/createdWith> <https://nanotate.bitsfetch.com> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#provenance> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#assertion> <http://www.w3.org/ns/prov#generatedAtTime> "2021-03-10T11:13:12.232860"^^<http://www.w3.org/2001/XMLSchema#dateTime> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#provenance> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#assertion> <http://www.w3.org/ns/prov#wasAttributedTo> <https://hypothes.is/users/alejandra.sandoval> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#provenance> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/5T5BtoFSEeuDVNszA62k8g> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#pubInfo> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/SsTfhoFTEeugz7M1dQr42w> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#pubInfo> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/XnU0XoFTEeu03pMhdlRcng> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#pubInfo> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/bKJEkIFTEeu4IH819XJVFw> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#pubInfo> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/fN60OIFTEeuXvTMD-qL4vw> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#pubInfo> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/mK8Z8IFTEeuvOSvYxUpUaw> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#pubInfo> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#sig> <http://purl.org/nanopub/x/hasAlgorithm> "RSA" <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#pubInfo> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#sig> <http://purl.org/nanopub/x/hasPublicKey> "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#pubInfo> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#sig> <http://purl.org/nanopub/x/hasSignature> "N4l+SnKO+1ioBbTttjSPHif+OJ3qBq7H/5m+yE5MQuX7xASSwc+U079Awu1kCXlNTooib3QoI5pdi/kklyNs4yFWOd6Ly8//iRnqNuf+44qRPezHNr8YJGSdX/JMKa3ZYrZ23cMju+zeWDT8+RQJwKpb8TtX27wbCQJbrJrHg+U=" <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#pubInfo> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#sig> <http://purl.org/nanopub/x/hasSignatureTarget> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#pubInfo> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ> <http://purl.org/nanopub/x/introduces> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#step> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#pubInfo> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ> <http://www.w3.org/ns/prov#generatedAtTime> "2021-03-10T11:13:12.232860"^^<http://www.w3.org/2001/XMLSchema#dateTime> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#pubInfo> .
<http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ> <http://www.w3.org/ns/prov#wasAttributedTo> <https://hypothes.is/users/alejandra.sandoval> <http://purl.org/np/RAvGvCVuXkuOv1MtbcatFQpneJ3BP8ev7wFHDKN2TYZBQ#pubInfo> .