http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#Head http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0 http://www.nanopub.org/nschema#hasAssertion http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#assertion http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0 http://www.nanopub.org/nschema#hasProvenance http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#provenance http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0 http://www.nanopub.org/nschema#hasPublicationInfo http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#pubInfo http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#assertion http://purl.obolibrary.org/obo/OBI_0002488 http://www.w3.org/2000/01/rdf-schema#label pipette http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#_1 http://www.w3.org/2000/01/rdf-schema#label mycobacteria http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#_2 http://www.w3.org/2000/01/rdf-schema#label mycobacteria http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#_3 http://www.w3.org/2000/01/rdf-schema#label culture medium http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#_4 http://www.w3.org/2000/01/rdf-schema#label TE http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#step http://purl.org/dc/elements/1.1/description Harvest mycobacteria by centrifuging 20-40 ml log phase culture (O.D.600 ≈ 0.8) at 2,000 x g for 10 min, room temperature. Carefully discard culture medium and add 10 ml TE (at room temperature) buffer to pellet. Mix gently with a pipette. Note: If isolating DNA from pathogenic mycobacteria such as M. tuberculosis, step 1 and 2 must be performed in a BSL-3 facility. http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#step http://purl.org/net/SMARTprotocol#hasExperimentalInput http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#_1 http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#step http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://purl.org/net/p-plan#Step http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#_2 http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed http://purl.obolibrary.org/obo/OBI_0002488 http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#_3 http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#_4 http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#provenance http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#assertion http://purl.org/pav/createdWith https://nanotate.bitsfetch.com http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#assertion http://www.w3.org/ns/prov#generatedAtTime 2021-03-10T11:20:30.406999 http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#assertion http://www.w3.org/ns/prov#wasAttributedTo https://hypothes.is/users/alejandra.sandoval http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#pubInfo http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/F5hHDoFQEeupMnfBwWNSFA http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/dko6BoFPEeuJf99nep5VFA http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/lwnWrIFPEeuSpOPhPMkNog http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/vFGivoFPEeu8CqN-Wci9Sw http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/zppHCoFPEeu7CktybqeS2Q http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#sig http://purl.org/nanopub/x/hasAlgorithm RSA http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#sig http://purl.org/nanopub/x/hasPublicKey MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#sig http://purl.org/nanopub/x/hasSignature MC6E9m3cxtSsdsvlva8NE1habj8YrPsLAxyw9icRRdO34BiwBHyie47jO85pkdrXP/3ghZ3ziJlfnMN+J/vgmDZToHG9kNd0ZDlCWGJZFurZ3PZCRwBp1TFKiVVs6gjP45/GBml+6vq0wvXyktenXZULNwqu+jADacA7WiyMW+U= http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#sig http://purl.org/nanopub/x/hasSignatureTarget http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0 http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0 http://purl.org/nanopub/x/introduces http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0#step http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0 http://www.w3.org/ns/prov#generatedAtTime 2021-03-10T11:20:30.406999 http://purl.org/np/RAu8IsZZpTdswXcqHkNw0hqJjkHlE3r30ya4v1lcoDVK0 http://www.w3.org/ns/prov#wasAttributedTo https://hypothes.is/users/alejandra.sandoval