@prefix this: <http://purl.org/np/RAtKnp_kibv4oYNO4QUDq2a3S7tz2rjOK9Aa2i646ECpY> .
@prefix sub: <http://purl.org/np/RAtKnp_kibv4oYNO4QUDq2a3S7tz2rjOK9Aa2i646ECpY#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/CHEBI_16991> rdfs:label "DNA" .
  
  <http://purl.obolibrary.org/obo/OBI_0000439> rdfs:label "agarose gel" .
  
  <http://purl.obolibrary.org/obo/OBI_0400115> rdfs:label "spectrophotometer" .
  
  sub:_1 rdfs:label "pellet" .
  
  sub:_2 rdfs:label "molecular biology grade water" .
  
  sub:step dc:description "Dissolve pellet in molecular biology grade water and analyze DNA by agarose gel electrophoresis and spectrophotometer for its purity and integrity.";
    sp:hasExperimentalOutput <http://purl.obolibrary.org/obo/CHEBI_16991>;
    a p-plan:Step;
    bs:bioSampleUsed <http://purl.obolibrary.org/obo/CHEBI_16991>, sub:_1;
    bs:labEquipmentUsed <http://purl.obolibrary.org/obo/OBI_0400115>;
    bs:reagentUsed <http://purl.obolibrary.org/obo/OBI_0000439>, sub:_2 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-02-26T09:39:38.488998"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:Kw3IuHhAEeugvC_qjxSBgA, authority:RarDNHg_EeupnnscZLetKg,
      authority:X9K7Ing_EeuO7n-Tus5eYA, authority:cBa3cng_EeuBtgsBD4MQRg, authority:ml0rzng_EeufVHf7O51lgw,
      authority:r-dENHg_EeuGqktNfgJ-3Q .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "JO31h2KdkoBVPOiK7yGJkS5af3J1kQMQKBNrtFzNUTdM8n5JhJAF8rCt7slhM+bCigR80XflKlNuLlsTnLu4pSEukJH8KTcOkno2jJdnn09RfeS8QurAQ22g+Lk1J7DR8sLu5/nNh/BWxOzk9zpsOMc5wIhEtAzFUQXaHG++oUs=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-02-26T09:39:38.488998"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}