@prefix this: . @prefix sub: . @prefix bs: . @prefix np: . @prefix pav: . @prefix authority: . @prefix npx: . @prefix xsd: . @prefix rdfs: . @prefix p-plan: . @prefix prov: . @prefix sp: . @prefix dc: . sub:Head { this: np:hasAssertion sub:assertion; np:hasProvenance sub:provenance; np:hasPublicationInfo sub:pubInfo; a np:Nanopublication . } sub:assertion { rdfs:label "DMEM" . sub:_1 rdfs:label "cells" . sub:_2 rdfs:label "cells" . sub:step dc:description """Wash cells (centrifuge at 100 x g for 10 min) with 10 ml of sterile DMEM (without FBS) twice. Note: Cold DMEM is not used routinely for washing lymphocytes from culture cavities while setting up cultures. Rather when monocytes bound tightly to plastic cavities are needed to be harvested pre-chilled DMEM can be used. """; sp:hasExperimentalOutput sub:_1; a p-plan:Step; bs:bioSampleUsed sub:_2; bs:reagentUsed . } sub:provenance { sub:assertion pav:createdWith ; prov:generatedAtTime "2021-03-05T10:08:35.231457"^^xsd:dateTime; prov:wasAttributedTo . } sub:pubInfo { sub:assertion prov:wasDerivedFrom authority:Iqikin0oEeuEobN3tukkMQ, authority:Pn1VKn0oEeut2T99emTI0A, authority:Ul6qxn0oEeufO-9TegxgZA . sub:sig npx:hasAlgorithm "RSA"; npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB"; npx:hasSignature "MaVfAfiTemYOYoX4V0FCGMP6Krh46NbiDyUU2qhr2iGn4DZRQY09gJryonoByj4nc6LWXT2b6CUz4q9KKk7+952Crb9CooP7upaYJ8WKeD8OoTjdpSJoktwUpT4OnC27D1nNTadqe11Pc3DeaykZSnAx0T2TjSvlWfxE6VAzJu4="; npx:hasSignatureTarget this: . this: npx:introduces sub:step; prov:generatedAtTime "2021-03-05T10:08:35.231457"^^xsd:dateTime; prov:wasAttributedTo . }