@prefix this: <http://purl.org/np/RAsJyZ5Znc54-tK37PmFveFPCA0USROZ7tO4XVMsraHW4> .
@prefix sub: <http://purl.org/np/RAsJyZ5Znc54-tK37PmFveFPCA0USROZ7tO4XVMsraHW4#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   <http://purl.obolibrary.org/obo/OBI_0000918> rdfs:label "DMEM" .
  sub:_1 rdfs:label "cells" .
  sub:_2 rdfs:label "cells" .
  sub:step dc:description """Wash cells (centrifuge at 100 x g for 10 min) with 10 ml of sterile DMEM (without FBS) twice.
Note: Cold DMEM is not used routinely for washing lymphocytes from culture cavities while setting up cultures. Rather when monocytes bound tightly to plastic cavities are needed to be harvested pre-chilled DMEM can be used.
    
    """ ;
    sp:hasExperimentalOutput sub:_1 ;
    a p-plan:Step ;
    bs:bioSampleUsed sub:_2 ;
    bs:reagentUsed <http://purl.obolibrary.org/obo/OBI_0000918> .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-03-05T10:08:35.231457"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:Iqikin0oEeuEobN3tukkMQ , authority:Pn1VKn0oEeut2T99emTI0A , authority:Ul6qxn0oEeufO-9TegxgZA .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "MaVfAfiTemYOYoX4V0FCGMP6Krh46NbiDyUU2qhr2iGn4DZRQY09gJryonoByj4nc6LWXT2b6CUz4q9KKk7+952Crb9CooP7upaYJ8WKeD8OoTjdpSJoktwUpT4OnC27D1nNTadqe11Pc3DeaykZSnAx0T2TjSvlWfxE6VAzJu4=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-03-05T10:08:35.231457"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}