http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4#Head http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4 http://www.nanopub.org/nschema#hasAssertion http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4#assertion http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4 http://www.nanopub.org/nschema#hasProvenance http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4#provenance http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4 http://www.nanopub.org/nschema#hasPublicationInfo http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4#pubInfo http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4#assertion http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4#_1 http://www.w3.org/2000/01/rdf-schema#label phenol:chloroform http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4#step http://purl.org/dc/elements/1.1/description Add equal volume of phenol:chloroform(1:1) ,shake the tube well for 5 minutes and centrifuge at 12,000g for 10 minutes at 4º celcius http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4#step http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://purl.org/net/p-plan#Step http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed http://www.bioassayontology.org/bao#BAO_0010014 http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4#_1 http://www.bioassayontology.org/bao#BAO_0010014 http://www.w3.org/2000/01/rdf-schema#label tube http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4#provenance http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4#assertion http://purl.org/pav/createdWith https://nanotate.bitsfetch.com http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4#assertion http://www.w3.org/ns/prov#generatedAtTime 2021-03-10T10:44:12.371337 http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4#assertion http://www.w3.org/ns/prov#wasAttributedTo https://hypothes.is/users/alejandra.sandoval http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4#pubInfo http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/FxWTUHJPEeufXltHJSP9JQ http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/aG4d0HJPEeuo9N-kjz6Aew http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/aYlpCoG3Eeu__asgFJ0hrg http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4#sig http://purl.org/nanopub/x/hasAlgorithm RSA http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4#sig http://purl.org/nanopub/x/hasPublicKey MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4#sig http://purl.org/nanopub/x/hasSignature FTz66PT0u8YnKbm1ZfS8SrzTrLrpztCaRQRYcTIXsfiuhTfZU8M136KpT4/MoHeCArh60srAX/BwybXeTuKzAOqwN3JBJoDYE1clOCra44EntQpY5agJJ5hqxyFIUxvoy6fvo2/DbHGeR4sevt0xvq/+zgtn+CTQu+Mdzx4EeG4= http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4#sig http://purl.org/nanopub/x/hasSignatureTarget http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4 http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4 http://purl.org/nanopub/x/introduces http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4#step http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4 http://www.w3.org/ns/prov#generatedAtTime 2021-03-10T10:44:12.371337 http://purl.org/np/RAr8FDnVJ5V1Z9vkijLYOrS2CBIWBuBHRhaX375jmQ6Q4 http://www.w3.org/ns/prov#wasAttributedTo https://hypothes.is/users/alejandra.sandoval