@prefix this: <http://purl.org/np/RAp4et_bMBAqEXfgEgIc3gADfsNTjCtPxx29vKzxWAZ6o> .
@prefix sub: <http://purl.org/np/RAp4et_bMBAqEXfgEgIc3gADfsNTjCtPxx29vKzxWAZ6o#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  sub:_1 rdfs:label "supernatant" .
  
  sub:_2 rdfs:label "2ml Eppendorf tubes" .
  
  sub:_3 rdfs:label "chloroform: isoamyl alcohol" .
  
  sub:step dc:description "Transfer 1ml of supernatant to each 2ml Eppendorf tubes already containing 1 ml of chloroform: isoamyl alcohol (24:1). Mix supernatant and chloroform: isoamyl alcohol by gentle inversions for 10 min and subsequently place the tube on ice for 10 min.";
    a p-plan:Step;
    bs:bioSampleUsed sub:_1;
    bs:labEquipmentUsed sub:_2;
    bs:reagentUsed sub:_3 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-02-25T16:26:43.935141"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:3Shw8HesEeuHNHd48NKrpw, authority:ls98_HesEeuKJ6Pkum6yJw,
      authority:p6dGmnesEeujz-sIYk4aAg, authority:xARpYnesEeuM9T-n5n4J8g .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "JyXNxHATCSsA1lhU8YZzed7D8i3NhP58WBcb8FdZwxj7PSV5aUcY4Esj6obv82KB3JBdYtQhsbI6t69T+5UifmgRmsoNcizCycaf0l3Ku47Yl1rVnAk7sgKvkTM8HPgP1IKoIzfA+tvllcZyzv3lZK9T6u/4txiiV1Ul/dseKBA=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-02-25T16:26:43.935141"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}