@prefix this: <http://purl.org/np/RAo_IfO6pT5evtX45h-18b0U4fAiTWFFBZ1kl3WuvfIqg> .
@prefix sub: <http://purl.org/np/RAo_IfO6pT5evtX45h-18b0U4fAiTWFFBZ1kl3WuvfIqg#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/CHEBI_16236> rdfs:label "95% ethanol" .
  
  <http://purl.obolibrary.org/obo/ERO_0000979> rdfs:label "-20°C freezer" .
  
  sub:_1 rdfs:label "tubes" .
  
  sub:step dc:description "Add 2 volumes of cold 95% ethanol and mix gently by inversion. The tubes are incubated at for 45 min in -20°C freezer. It should not be for more time as some remaining phenolics and resin may also precipitate with DNA.";
    a p-plan:Step;
    bs:labEquipmentUsed <http://purl.obolibrary.org/obo/ERO_0000979>, sub:_1;
    bs:reagentUsed <http://purl.obolibrary.org/obo/CHEBI_16236> .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-02-25T16:20:43.266935"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom <https://hypothes.is/a/-j006netEeu3IvO1mTUuxA>,
      authority:Dv5_4neuEeuPNDMtI9XS8A, authority:Jb-EOHeuEeu83atKs8uF9w, authority:P12A_HeuEeuHOWeTiYtpDA .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "HFPjAjTWEz0Nlf9Flp8ZEsZKbFW4uUYoREpAwxQ7cscMpRrQmkpsEm5Jiz09hjxorM+nS+u5KqqqNzsMwIghAaW6uJIrNNgecRwp0lIpJqTKH2P83w5VDv61JA1aTyPPksnQrV/nTza+g4uqqf45c5GYk6Z2ScbLJGGhT1F3qWE=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-02-25T16:20:43.266935"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}