. . . . " root" . "leaf" . "recalcitrant samples" . "recalcitrant samples" . "mortar" . "pestle" . "liquid nitrogen" . "extraction buffer" . "Ground samples (leaf, shoot, root, and recalcitrant samples, approximately 0.5-1 g) using 1 ml of the extraction buffer with or without liquid nitrogen in mortar and pestle that are sterilized.CRITICAL STEP The procedure are carried out at room temperature except the centrifugation steps (at 4℃) as well as the time of precipitating of the nucleic acid using the isopropanol (on ice).CRITICAL STEP Severely disrupt the tissues to create the glaze mode of samples" . . . . . . . . . . . . . . "shoot" . . "2021-03-10T10:16:14.459970"^^ . . . . . . . . . . . "RSA" . "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" . "ICqufBq+ZemZiQADI6AET0h71gC/UtyHkeZPzACNzGkuNzmXT69sxGKqpfZV4YFhsKdrEAQWMJXjXLPduFMw0zvDRsX8EDsMPw20h1dyc0QCA8fzI3MddHlpxswu64ZRkLz57LbcKeiEZh0uXZ9SkDplbo/eM0UUGgESw6u/j1Q=" . . . "2021-03-10T10:16:14.459970"^^ . .