@prefix this: <http://purl.org/np/RAhVgJ51J0tj19e4Xwsx-pLjzfUStEnsZH1k5NFydCi1Q> .
@prefix sub: <http://purl.org/np/RAhVgJ51J0tj19e4Xwsx-pLjzfUStEnsZH1k5NFydCi1Q#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
sub:Head {
  this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
  <http://purl.obolibrary.org/obo/CHEBI_32954> rdfs:label "sodium acetate solution" .
  sub:_1 rdfs:label "aqueous phase" .
  sub:step dc:description "Add 100 µl 3 M sodium acetate solution per ml of aqueous phase obtained in step 10. Mix gently by inverting tube." ;
    a p-plan:Step ;
    bs:bioSampleUsed sub:_1 ;
    bs:labEquipmentUsed <http://www.bioassayontology.org/bao#BAO_0010014> ;
    bs:reagentUsed <http://purl.obolibrary.org/obo/CHEBI_32954> .
  <http://www.bioassayontology.org/bao#BAO_0010014> rdfs:label "tube" .
}
sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-02-26T09:49:12.595345"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}
sub:pubInfo {
  sub:assertion prov:wasDerivedFrom <https://hypothes.is/a/-YEIKng9EeucUqfQGzAgNg> , authority:2kRDUHg9EeuRGKNody3Xug , authority:DgmQUHg-EeufxCPQLP2Hlg , authority:JSGjmng-EeurH9uOlP3kmg .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "e736GcEmooFXHfgh6TLkR1ER6bIDYNGRK38+435B5j/IhDtinzBQNEgElaHu1O7TKVUlRK1yPdmR4Ga0rz2WspFUuCURdk4NeZuBrcMnIfg3gGRr+oRdByd3DeA8DWDtCNKzkhpoV1BCQORHF3fSVOfVfMc1FDAiTSVyTylLfLs=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-02-26T09:49:12.595345"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}