@prefix this: <http://purl.org/np/RAgJjjShkAgDJRhJ4KeEWjk2jxUkfUfuY5d-2UOUxv_NQ> .
@prefix sub: <http://purl.org/np/RAgJjjShkAgDJRhJ4KeEWjk2jxUkfUfuY5d-2UOUxv_NQ#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/OBI_0000918> rdfs:label "Dulbecco's modified eagle medium" .
  
  sub:_1 rdfs:label "PBS" .
  
  sub:step dc:description "Wash (centrifuge in 100 x g for 10 min) twice with 10 ml of sterile PBS or sterile Dulbecco's modified eagle medium. The approximate yield of cells from 4 ml of blood varies between 107-108.";
    a p-plan:Step;
    bs:reagentUsed <http://purl.obolibrary.org/obo/OBI_0000918>, sub:_1 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-23T16:30:58.156283"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:2afD4H0kEeuoodflsCE7FQ, authority:7nio8n0kEeuKhkPrnYUA5Q,
      authority:AUysOn0lEeuWHyNH4JgzbA .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "YbHvsUvgDLbjsYmBDdxIihVrES1NtXa3clAgOmB1WSQNi11E+S8V3lg4FQdqZSrRfhyxP1CgLSSifyH2Cxc2wxxOPtnk8D7jOTkrg+u6Udrca6YkMbWvi8ERqxPybxcHzcGpgoxSfVIQMTqBfoyOc8hDYUuS8DSXeKL7dumGJjs=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-23T16:30:58.156283"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}