[ {
  "@graph" : [ {
    "@id" : "http://purl.org/np/RAgJjjShkAgDJRhJ4KeEWjk2jxUkfUfuY5d-2UOUxv_NQ",
    "@type" : [ "http://www.nanopub.org/nschema#Nanopublication" ],
    "http://www.nanopub.org/nschema#hasAssertion" : [ {
      "@id" : "http://purl.org/np/RAgJjjShkAgDJRhJ4KeEWjk2jxUkfUfuY5d-2UOUxv_NQ#assertion"
    } ],
    "http://www.nanopub.org/nschema#hasProvenance" : [ {
      "@id" : "http://purl.org/np/RAgJjjShkAgDJRhJ4KeEWjk2jxUkfUfuY5d-2UOUxv_NQ#provenance"
    } ],
    "http://www.nanopub.org/nschema#hasPublicationInfo" : [ {
      "@id" : "http://purl.org/np/RAgJjjShkAgDJRhJ4KeEWjk2jxUkfUfuY5d-2UOUxv_NQ#pubInfo"
    } ]
  } ],
  "@id" : "http://purl.org/np/RAgJjjShkAgDJRhJ4KeEWjk2jxUkfUfuY5d-2UOUxv_NQ#Head"
}, {
  "@graph" : [ {
    "@id" : "http://purl.obolibrary.org/obo/OBI_0000918",
    "http://www.w3.org/2000/01/rdf-schema#label" : [ {
      "@value" : "Dulbecco's modified eagle medium"
    } ]
  }, {
    "@id" : "http://purl.org/np/RAgJjjShkAgDJRhJ4KeEWjk2jxUkfUfuY5d-2UOUxv_NQ#_1",
    "http://www.w3.org/2000/01/rdf-schema#label" : [ {
      "@value" : "PBS"
    } ]
  }, {
    "@id" : "http://purl.org/np/RAgJjjShkAgDJRhJ4KeEWjk2jxUkfUfuY5d-2UOUxv_NQ#step",
    "@type" : [ "http://purl.org/net/p-plan#Step" ],
    "http://purl.org/dc/elements/1.1/description" : [ {
      "@value" : "Wash (centrifuge in 100 x g for 10 min) twice with 10 ml of sterile PBS or sterile Dulbecco's modified eagle medium. The approximate yield of cells from 4 ml of blood varies between 107-108."
    } ],
    "https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed" : [ {
      "@id" : "http://purl.obolibrary.org/obo/OBI_0000918"
    }, {
      "@id" : "http://purl.org/np/RAgJjjShkAgDJRhJ4KeEWjk2jxUkfUfuY5d-2UOUxv_NQ#_1"
    } ]
  } ],
  "@id" : "http://purl.org/np/RAgJjjShkAgDJRhJ4KeEWjk2jxUkfUfuY5d-2UOUxv_NQ#assertion"
}, {
  "@graph" : [ {
    "@id" : "http://purl.org/np/RAgJjjShkAgDJRhJ4KeEWjk2jxUkfUfuY5d-2UOUxv_NQ#assertion",
    "http://purl.org/pav/createdWith" : [ {
      "@id" : "https://nanotate.bitsfetch.com"
    } ],
    "http://www.w3.org/ns/prov#generatedAtTime" : [ {
      "@type" : "http://www.w3.org/2001/XMLSchema#dateTime",
      "@value" : "2021-03-23T16:30:58.156283"
    } ],
    "http://www.w3.org/ns/prov#wasAttributedTo" : [ {
      "@id" : "https://hypothes.is/users/iannotate"
    } ]
  } ],
  "@id" : "http://purl.org/np/RAgJjjShkAgDJRhJ4KeEWjk2jxUkfUfuY5d-2UOUxv_NQ#provenance"
}, {
  "@graph" : [ {
    "@id" : "http://purl.org/np/RAgJjjShkAgDJRhJ4KeEWjk2jxUkfUfuY5d-2UOUxv_NQ",
    "http://purl.org/nanopub/x/introduces" : [ {
      "@id" : "http://purl.org/np/RAgJjjShkAgDJRhJ4KeEWjk2jxUkfUfuY5d-2UOUxv_NQ#step"
    } ],
    "http://www.w3.org/ns/prov#generatedAtTime" : [ {
      "@type" : "http://www.w3.org/2001/XMLSchema#dateTime",
      "@value" : "2021-03-23T16:30:58.156283"
    } ],
    "http://www.w3.org/ns/prov#wasAttributedTo" : [ {
      "@id" : "https://hypothes.is/users/iannotate"
    } ]
  }, {
    "@id" : "http://purl.org/np/RAgJjjShkAgDJRhJ4KeEWjk2jxUkfUfuY5d-2UOUxv_NQ#assertion",
    "http://www.w3.org/ns/prov#wasDerivedFrom" : [ {
      "@id" : "https://hypothes.is/a/2afD4H0kEeuoodflsCE7FQ"
    }, {
      "@id" : "https://hypothes.is/a/7nio8n0kEeuKhkPrnYUA5Q"
    }, {
      "@id" : "https://hypothes.is/a/AUysOn0lEeuWHyNH4JgzbA"
    } ]
  }, {
    "@id" : "http://purl.org/np/RAgJjjShkAgDJRhJ4KeEWjk2jxUkfUfuY5d-2UOUxv_NQ#sig",
    "http://purl.org/nanopub/x/hasAlgorithm" : [ {
      "@value" : "RSA"
    } ],
    "http://purl.org/nanopub/x/hasPublicKey" : [ {
      "@value" : "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB"
    } ],
    "http://purl.org/nanopub/x/hasSignature" : [ {
      "@value" : "YbHvsUvgDLbjsYmBDdxIihVrES1NtXa3clAgOmB1WSQNi11E+S8V3lg4FQdqZSrRfhyxP1CgLSSifyH2Cxc2wxxOPtnk8D7jOTkrg+u6Udrca6YkMbWvi8ERqxPybxcHzcGpgoxSfVIQMTqBfoyOc8hDYUuS8DSXeKL7dumGJjs="
    } ],
    "http://purl.org/nanopub/x/hasSignatureTarget" : [ {
      "@id" : "http://purl.org/np/RAgJjjShkAgDJRhJ4KeEWjk2jxUkfUfuY5d-2UOUxv_NQ"
    } ]
  } ],
  "@id" : "http://purl.org/np/RAgJjjShkAgDJRhJ4KeEWjk2jxUkfUfuY5d-2UOUxv_NQ#pubInfo"
} ]