@prefix this: <http://purl.org/np/RAea1HarqPKjTqaYCgFAZIHurIPMDCvVfekwWQBiPEv_g> .
@prefix sub: <http://purl.org/np/RAea1HarqPKjTqaYCgFAZIHurIPMDCvVfekwWQBiPEv_g#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/CHEBI_16991> rdfs:label "DNA" .
  
  <http://purl.obolibrary.org/obo/CHEBI_17824> rdfs:label " isopropanol" .
  
  sub:step dc:description "Add an equal volume of isopropanol to tube. Mix contents gently and keep undisturbed for 5-10 min. The white thread-like structure of precipitated DNA will be seen in suspension";
    sp:hasExperimentalOutput <http://purl.obolibrary.org/obo/CHEBI_16991>;
    a p-plan:Step;
    bs:bioSampleUsed <http://purl.obolibrary.org/obo/CHEBI_16991>;
    bs:labEquipmentUsed <http://www.bioassayontology.org/bao#BAO_0010014>;
    bs:reagentUsed <http://purl.obolibrary.org/obo/CHEBI_17824> .
  
  <http://www.bioassayontology.org/bao#BAO_0010014> rdfs:label "tube" .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-02-26T09:46:53.028236"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:5c6XWng_EeufV4Mf5WmEkA, authority:PR5n5Hg-EeusjNPPSRf4PQ,
      authority:U-5CRng-EeuRLaefFZBjvg, authority:d0B2dHg-EeufUIP78yBROQ .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "b47qol9W+RwuLX1SAOsGtadNeonj0pZTNtNV+k5dB71Oyu9CKFOE98AqBL3ZOct4pKGO2+eHVMwhY6CvC2lHiZ9HwKInsW+NqDC9NdCI9B9fq3rLobmqNeZi3//sIQrnGTO3oopqzS3n/0CFcsdLqq1o3puvE8wBLiBZ85855/Y=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-02-26T09:46:53.028236"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}