[ { "@graph" : [ { "@id" : "http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ", "@type" : [ "http://www.nanopub.org/nschema#Nanopublication" ], "http://www.nanopub.org/nschema#hasAssertion" : [ { "@id" : "http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ#assertion" } ], "http://www.nanopub.org/nschema#hasProvenance" : [ { "@id" : "http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ#provenance" } ], "http://www.nanopub.org/nschema#hasPublicationInfo" : [ { "@id" : "http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ#pubInfo" } ] } ], "@id" : "http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ#Head" }, { "@graph" : [ { "@id" : "http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C62218", "http://www.w3.org/2000/01/rdf-schema#label" : [ { "@value" : "70% ethanol" } ] }, { "@id" : "http://purl.obolibrary.org/obo/CHEBI_16991", "http://www.w3.org/2000/01/rdf-schema#label" : [ { "@value" : "DNA" } ] }, { "@id" : "http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ#_1", "http://www.w3.org/2000/01/rdf-schema#label" : [ { "@value" : "pellet" } ] }, { "@id" : "http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ#_2", "http://www.w3.org/2000/01/rdf-schema#label" : [ { "@value" : "tubes" } ] }, { "@id" : "http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ#_3", "http://www.w3.org/2000/01/rdf-schema#label" : [ { "@value" : "TE" } ] }, { "@id" : "http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ#step", "@type" : [ "http://purl.org/net/p-plan#Step" ], "http://purl.org/dc/elements/1.1/description" : [ { "@value" : "After incubation, the tubes were centrifuged at 5000 × g for 10 min at 4°C and the supernatant was gently removed. The pellet is washed two times with 1ml of 70% ethanol and the DNA is pellet by 5000 × g at 4°C for only 5 min. The supernatant is discarded and the pellet is air-dried (10 min). The pellet are allowed to re-suspend in 50 μL of TE (10 mM Tris. HCl pH 8.0; 1 mM EDTA pH 8.0)." } ], "http://purl.org/net/SMARTprotocol#hasExperimentalOutput" : [ { "@id" : "http://purl.obolibrary.org/obo/CHEBI_16991" } ], "https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed" : [ { "@id" : "http://purl.obolibrary.org/obo/CHEBI_16991" }, { "@id" : "http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ#_1" } ], "https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed" : [ { "@id" : "http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ#_2" } ], "https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed" : [ { "@id" : "http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C62218" }, { "@id" : "http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ#_3" } ] } ], "@id" : "http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ#assertion" }, { "@graph" : [ { "@id" : "http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ#assertion", "http://purl.org/pav/createdWith" : [ { "@id" : "https://nanotate.bitsfetch.com" } ], "http://www.w3.org/ns/prov#generatedAtTime" : [ { "@type" : "http://www.w3.org/2001/XMLSchema#dateTime", "@value" : "2021-03-11T16:39:49.461208" } ], "http://www.w3.org/ns/prov#wasAttributedTo" : [ { "@id" : "https://hypothes.is/users/iannotate" } ] } ], "@id" : "http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ#provenance" }, { "@graph" : [ { "@id" : "http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ", "http://purl.org/nanopub/x/introduces" : [ { "@id" : "http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ#step" } ], "http://www.w3.org/ns/prov#generatedAtTime" : [ { "@type" : "http://www.w3.org/2001/XMLSchema#dateTime", "@value" : "2021-03-11T16:39:49.461208" } ], "http://www.w3.org/ns/prov#wasAttributedTo" : [ { "@id" : "https://hypothes.is/users/iannotate" } ] }, { "@id" : "http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ#assertion", "http://www.w3.org/ns/prov#wasDerivedFrom" : [ { "@id" : "https://hypothes.is/a/-I88TIKxEeu85fuKGtcBIw" }, { "@id" : "https://hypothes.is/a/4n20poKxEeu9i_eN_Ca7Dw" }, { "@id" : "https://hypothes.is/a/DkWQSoKyEeuQBW-OJG3rAQ" }, { "@id" : "https://hypothes.is/a/KTUEYoKyEeuz26fBERP8ow" }, { "@id" : "https://hypothes.is/a/u6u4PIKxEeuEqgdWCyMzjQ" }, { "@id" : "https://hypothes.is/a/zsdcWoKxEeun64_lb_lpTg" } ] }, { "@id" : "http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ#sig", "http://purl.org/nanopub/x/hasAlgorithm" : [ { "@value" : "RSA" } ], "http://purl.org/nanopub/x/hasPublicKey" : [ { "@value" : "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" } ], "http://purl.org/nanopub/x/hasSignature" : [ { "@value" : "WSksEo+95kLDcq4ezOI8iCMgFiMU4KLiLWhZquNxfHGJDW5UHUogYpmP0TczC4QUlpJC92X4k0iD2qF6sn6bETrWDeLagvjsZgE8MGESraGLGkK8pAoSnewc5icmi28O2jXUqNMjBCNRa1RmQmQjSVqXQFM/Im6mZWaf8gZIdGM=" } ], "http://purl.org/nanopub/x/hasSignatureTarget" : [ { "@id" : "http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ" } ] } ], "@id" : "http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ#pubInfo" } ]