@prefix this: <http://purl.org/np/RAeOFWKOl2SCIWtSVdjzhQje1i_zRbHSFstFml1qbzexE> .
@prefix sub: <http://purl.org/np/RAeOFWKOl2SCIWtSVdjzhQje1i_zRbHSFstFml1qbzexE#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  sub:_1 rdfs:label "cells" .
  
  sub:_2 rdfs:label "TRIzol" .
  
  sub:step dc:description "At the indicated time points (0, 2, 4, 8, 12, 16, 20, 24, 28 h post-infection), cells were rinsed with 10 ml Phosphate Buffered Saline (PBS) buffer once and lysed in 1 ml TRIzol for 5 min at room temperature.";
    a p-plan:Step;
    bs:bioSampleUsed sub:_1;
    bs:reagentUsed sub:_2, <http://www.bioassayontology.org/bao#BAO_0000012> .
  
  <http://www.bioassayontology.org/bao#BAO_0000012> rdfs:label "Phosphate Buffered Saline (PBS)" .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-02-18T09:45:01.698523"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:1Ffz1HE-Eeu1uMvEPxCSkw, authority:cj4GXHE-EeuIGHOi25ZRTw,
      authority:uwpLKnE-EeuYS4etSXHzcQ, authority:xoEKUnH3EeuYACNYb49-Qw .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "AmXNE0N2OMGwXS7neBjYbbJSzsSoBqLEjxB/bp6qGgs01keBgXqWqoNjOlIh238g+Y7PWn8W07QckUCmjKwbC42aR7rr8zRUzFtE3+sIQOawG0b7im/ccAH4vYMls8E7eos8V4ofTrKV6J4fahXTFUA72PHcSwW5Z/ISJqUr308=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-02-18T09:45:01.698523"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}