@prefix this: <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU> .
@prefix sub: <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/CHEBI_17824> rdfs:label "isopropanol" .
  
  sub:_1 rdfs:label "supernatant" .
  
  sub:step dc:description "Transfer the very clear supernatant to a fresh tube , add two fold volume cold  isopropanol and keep it for 1 hour at -20º celcius  .";
    a p-plan:Step;
    bs:bioSampleUsed sub:_1;
    bs:labEquipmentUsed <http://www.bioassayontology.org/bao#BAO_0010014>;
    bs:reagentUsed <http://purl.obolibrary.org/obo/CHEBI_17824> .
  
  <http://www.bioassayontology.org/bao#BAO_0010014> rdfs:label "tube" .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-11T10:49:31.220242"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:0wMwCoJ_Eeuy-YtFFDmZcg, authority:ki49uIJ_Eeun4a_ST-REGg,
      authority:plECqIJ_EeuG_3dY8FAfWQ, authority:uC576oJ_Eeus-48-dZ4sOA .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "DzvyxAGjYKwm3DKVt8228SlBXDnP9ZLk5f0PmnS2Iq9lDktk8wxGBsifxH4Ugmy1rDsx3xuEsBCfLWUHFAMWIz/5Z3jGXyqrJbWMBRdPBDwl3xYpxb7bTPNI3K+oaQYN6IsPup2L0H0feAlQmTZ2CIvYUcUL7+/L5E+jvvUc3pE=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-11T10:49:31.220242"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}