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<http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU> <http://www.nanopub.org/nschema#hasProvenance> <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#provenance> <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#Head> .
<http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU> <http://www.nanopub.org/nschema#hasPublicationInfo> <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#pubInfo> <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#Head> .
<http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://www.nanopub.org/nschema#Nanopublication> <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#Head> .
<http://purl.obolibrary.org/obo/CHEBI_17824> <http://www.w3.org/2000/01/rdf-schema#label> "isopropanol" <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#assertion> .
<http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#_1> <http://www.w3.org/2000/01/rdf-schema#label> "supernatant" <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#assertion> .
<http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#step> <http://purl.org/dc/elements/1.1/description> "Transfer the very clear supernatant to a fresh tube , add two fold volume cold  isopropanol and keep it for 1 hour at -20º celcius  ." <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#assertion> .
<http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#step> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/net/p-plan#Step> <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#assertion> .
<http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#step> <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed> <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#_1> <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#assertion> .
<http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#step> <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed> <http://www.bioassayontology.org/bao#BAO_0010014> <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#assertion> .
<http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#step> <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed> <http://purl.obolibrary.org/obo/CHEBI_17824> <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#assertion> .
<http://www.bioassayontology.org/bao#BAO_0010014> <http://www.w3.org/2000/01/rdf-schema#label> "tube" <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#assertion> .
<http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#assertion> <http://purl.org/pav/createdWith> <https://nanotate.bitsfetch.com> <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#provenance> .
<http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#assertion> <http://www.w3.org/ns/prov#generatedAtTime> "2021-03-11T10:49:31.220242"^^<http://www.w3.org/2001/XMLSchema#dateTime> <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#provenance> .
<http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#assertion> <http://www.w3.org/ns/prov#wasAttributedTo> <https://hypothes.is/users/iannotate> <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#provenance> .
<http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/0wMwCoJ_Eeuy-YtFFDmZcg> <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#pubInfo> .
<http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/ki49uIJ_Eeun4a_ST-REGg> <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#pubInfo> .
<http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/plECqIJ_EeuG_3dY8FAfWQ> <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#pubInfo> .
<http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/uC576oJ_Eeus-48-dZ4sOA> <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#pubInfo> .
<http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#sig> <http://purl.org/nanopub/x/hasAlgorithm> "RSA" <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#pubInfo> .
<http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#sig> <http://purl.org/nanopub/x/hasPublicKey> "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#pubInfo> .
<http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#sig> <http://purl.org/nanopub/x/hasSignature> "DzvyxAGjYKwm3DKVt8228SlBXDnP9ZLk5f0PmnS2Iq9lDktk8wxGBsifxH4Ugmy1rDsx3xuEsBCfLWUHFAMWIz/5Z3jGXyqrJbWMBRdPBDwl3xYpxb7bTPNI3K+oaQYN6IsPup2L0H0feAlQmTZ2CIvYUcUL7+/L5E+jvvUc3pE=" <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#pubInfo> .
<http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#sig> <http://purl.org/nanopub/x/hasSignatureTarget> <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU> <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#pubInfo> .
<http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU> <http://purl.org/nanopub/x/introduces> <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#step> <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#pubInfo> .
<http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU> <http://www.w3.org/ns/prov#generatedAtTime> "2021-03-11T10:49:31.220242"^^<http://www.w3.org/2001/XMLSchema#dateTime> <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#pubInfo> .
<http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU> <http://www.w3.org/ns/prov#wasAttributedTo> <https://hypothes.is/users/iannotate> <http://purl.org/np/RAaA8mdWnF4VnuN9AVhYKiKnsSMD6UDHV6-Vw-A5ECsaU#pubInfo> .