@prefix this: <http://purl.org/np/RA_YF4ofhZI0leT3rK8aCWbOyf9byQvyueEVBnQIhSM0M> .
@prefix sub: <http://purl.org/np/RA_YF4ofhZI0leT3rK8aCWbOyf9byQvyueEVBnQIhSM0M#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/OBI_0400155> rdfs:label "water bath" .
  
  sub:_1 rdfs:label "10 mg/ml Proteinase K" .
  
  sub:_2 rdfs:label "10% SDS" .
  
  sub:step dc:description "Add 1 ml of 10% SDS solution (final concentration, ~1%) and 20 µl of 10 mg/ml Proteinase K solution (final concentration 20 µg/ml). Incubate for 2 to 4 h at 60 °C in a hot water bath";
    a p-plan:Step;
    bs:labEquipmentUsed <http://purl.obolibrary.org/obo/OBI_0400155>;
    bs:reagentUsed sub:_1, sub:_2 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-10T11:10:06.349433"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:Ea2WaoFUEeuFL4MGeIWhCQ, authority:JyQj7IFUEeuCgo9Wp-9ZjA,
      authority:VTQhxIFUEeuDY6si7cuOXw, authority:a9-LNIFUEeuM1mNq5wVeCw .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "GwdW5eHEeDGw4VbKDO0wb3y74gO9bxdEhRlJKSOJ3404OgWRMCG3aUpDTvqDoYGQpLXWQ4FI1VodfwRJE/7W3YeLVDFDy8L870Qzmr8OvY3mQChP6DYlvTxuAEgugm3+pRbYdG29rhPu3uKWPOsWxE8555ZnwJmHyxYVZpCaLaA=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-10T11:10:06.349433"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}