[ { "@graph" : [ { "@id" : "http://purl.org/np/RA_YF4ofhZI0leT3rK8aCWbOyf9byQvyueEVBnQIhSM0M", "@type" : [ "http://www.nanopub.org/nschema#Nanopublication" ], "http://www.nanopub.org/nschema#hasAssertion" : [ { "@id" : "http://purl.org/np/RA_YF4ofhZI0leT3rK8aCWbOyf9byQvyueEVBnQIhSM0M#assertion" } ], "http://www.nanopub.org/nschema#hasProvenance" : [ { "@id" : "http://purl.org/np/RA_YF4ofhZI0leT3rK8aCWbOyf9byQvyueEVBnQIhSM0M#provenance" } ], "http://www.nanopub.org/nschema#hasPublicationInfo" : [ { "@id" : "http://purl.org/np/RA_YF4ofhZI0leT3rK8aCWbOyf9byQvyueEVBnQIhSM0M#pubInfo" } ] } ], "@id" : "http://purl.org/np/RA_YF4ofhZI0leT3rK8aCWbOyf9byQvyueEVBnQIhSM0M#Head" }, { "@graph" : [ { "@id" : "http://purl.obolibrary.org/obo/OBI_0400155", "http://www.w3.org/2000/01/rdf-schema#label" : [ { "@value" : "water bath" } ] }, { "@id" : "http://purl.org/np/RA_YF4ofhZI0leT3rK8aCWbOyf9byQvyueEVBnQIhSM0M#_1", "http://www.w3.org/2000/01/rdf-schema#label" : [ { "@value" : "10 mg/ml Proteinase K" } ] }, { "@id" : "http://purl.org/np/RA_YF4ofhZI0leT3rK8aCWbOyf9byQvyueEVBnQIhSM0M#_2", "http://www.w3.org/2000/01/rdf-schema#label" : [ { "@value" : "10% SDS" } ] }, { "@id" : "http://purl.org/np/RA_YF4ofhZI0leT3rK8aCWbOyf9byQvyueEVBnQIhSM0M#step", "@type" : [ "http://purl.org/net/p-plan#Step" ], "http://purl.org/dc/elements/1.1/description" : [ { "@value" : "Add 1 ml of 10% SDS solution (final concentration, ~1%) and 20 µl of 10 mg/ml Proteinase K solution (final concentration 20 µg/ml). Incubate for 2 to 4 h at 60 °C in a hot water bath" } ], "https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed" : [ { "@id" : "http://purl.obolibrary.org/obo/OBI_0400155" } ], "https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed" : [ { "@id" : "http://purl.org/np/RA_YF4ofhZI0leT3rK8aCWbOyf9byQvyueEVBnQIhSM0M#_1" }, { "@id" : "http://purl.org/np/RA_YF4ofhZI0leT3rK8aCWbOyf9byQvyueEVBnQIhSM0M#_2" } ] } ], "@id" : "http://purl.org/np/RA_YF4ofhZI0leT3rK8aCWbOyf9byQvyueEVBnQIhSM0M#assertion" }, { "@graph" : [ { "@id" : "http://purl.org/np/RA_YF4ofhZI0leT3rK8aCWbOyf9byQvyueEVBnQIhSM0M#assertion", "http://purl.org/pav/createdWith" : [ { "@id" : "https://nanotate.bitsfetch.com" } ], "http://www.w3.org/ns/prov#generatedAtTime" : [ { "@type" : "http://www.w3.org/2001/XMLSchema#dateTime", "@value" : "2021-03-10T11:10:06.349433" } ], "http://www.w3.org/ns/prov#wasAttributedTo" : [ { "@id" : "https://hypothes.is/users/alejandra.sandoval" } ] } ], "@id" : "http://purl.org/np/RA_YF4ofhZI0leT3rK8aCWbOyf9byQvyueEVBnQIhSM0M#provenance" }, { "@graph" : [ { "@id" : "http://purl.org/np/RA_YF4ofhZI0leT3rK8aCWbOyf9byQvyueEVBnQIhSM0M", "http://purl.org/nanopub/x/introduces" : [ { "@id" : "http://purl.org/np/RA_YF4ofhZI0leT3rK8aCWbOyf9byQvyueEVBnQIhSM0M#step" } ], "http://www.w3.org/ns/prov#generatedAtTime" : [ { "@type" : "http://www.w3.org/2001/XMLSchema#dateTime", "@value" : "2021-03-10T11:10:06.349433" } ], "http://www.w3.org/ns/prov#wasAttributedTo" : [ { "@id" : "https://hypothes.is/users/alejandra.sandoval" } ] }, { "@id" : "http://purl.org/np/RA_YF4ofhZI0leT3rK8aCWbOyf9byQvyueEVBnQIhSM0M#assertion", "http://www.w3.org/ns/prov#wasDerivedFrom" : [ { "@id" : "https://hypothes.is/a/Ea2WaoFUEeuFL4MGeIWhCQ" }, { "@id" : "https://hypothes.is/a/JyQj7IFUEeuCgo9Wp-9ZjA" }, { "@id" : "https://hypothes.is/a/VTQhxIFUEeuDY6si7cuOXw" }, { "@id" : "https://hypothes.is/a/a9-LNIFUEeuM1mNq5wVeCw" } ] }, { "@id" : "http://purl.org/np/RA_YF4ofhZI0leT3rK8aCWbOyf9byQvyueEVBnQIhSM0M#sig", "http://purl.org/nanopub/x/hasAlgorithm" : [ { "@value" : "RSA" } ], "http://purl.org/nanopub/x/hasPublicKey" : [ { "@value" : "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" } ], "http://purl.org/nanopub/x/hasSignature" : [ { "@value" : "GwdW5eHEeDGw4VbKDO0wb3y74gO9bxdEhRlJKSOJ3404OgWRMCG3aUpDTvqDoYGQpLXWQ4FI1VodfwRJE/7W3YeLVDFDy8L870Qzmr8OvY3mQChP6DYlvTxuAEgugm3+pRbYdG29rhPu3uKWPOsWxE8555ZnwJmHyxYVZpCaLaA=" } ], "http://purl.org/nanopub/x/hasSignatureTarget" : [ { "@id" : "http://purl.org/np/RA_YF4ofhZI0leT3rK8aCWbOyf9byQvyueEVBnQIhSM0M" } ] } ], "@id" : "http://purl.org/np/RA_YF4ofhZI0leT3rK8aCWbOyf9byQvyueEVBnQIhSM0M#pubInfo" } ]