@prefix this: <http://purl.org/np/RAYlfalu4NYTdo5BQb208vgT8dTz4sa36yT9Oyz3Dupgo> .
@prefix sub: <http://purl.org/np/RAYlfalu4NYTdo5BQb208vgT8dTz4sa36yT9Oyz3Dupgo#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/CHEBI_35255> rdfs:label "chloroform" .
  
  sub:_1 rdfs:label "Cell lysates" .
  
  sub:_2 rdfs:label "eppendorf tubes" .
  
  sub:step dc:description """Cell lysates were transfer into eppendorf tubes and one-fifth (volume/volume) of chloroform was added to each tube. 
			
			""";
    a p-plan:Step;
    bs:bioSampleUsed sub:_1;
    bs:labEquipmentUsed sub:_2;
    bs:reagentUsed <http://purl.obolibrary.org/obo/CHEBI_35255> .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-05T15:51:24.151487"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:7hg5CH3yEeu3hos30xrfbQ, authority:PZxP8n3xEeus0DOOXAgDNQ,
      authority:T2QAaH3xEeu41_eyD0wFmA, authority:Yd4czn3xEeun_z9u4wYUhQ .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "b6LiH+YmFyF2NbxDmMhJMDy/s5GfRh9MWxtfweV0XFw4q0OGdzJgQ9rG3RlQWOg0dx4eY0X+H4VO6enqk+Q3wDNVTd0AKKKJjJ1KO+j8TARF572vxWyrZamq4e2bL8bpiPrRL+REF+ubjffISk9g3DznEAlqaYfKfpZUcLrlDNw=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-05T15:51:24.151487"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}