http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#Head http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo http://www.nanopub.org/nschema#hasAssertion http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#assertion http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo http://www.nanopub.org/nschema#hasProvenance http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#provenance http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo http://www.nanopub.org/nschema#hasPublicationInfo http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#pubInfo http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#assertion http://purl.obolibrary.org/obo/PO_0009005 http://www.w3.org/2000/01/rdf-schema#label root http://purl.obolibrary.org/obo/PO_0025034 http://www.w3.org/2000/01/rdf-schema#label leaf http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#_1 http://www.w3.org/2000/01/rdf-schema#label recalcitrant samples http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#_2 http://www.w3.org/2000/01/rdf-schema#label recalcitrant samples http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#_3 http://www.w3.org/2000/01/rdf-schema#label mortar http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#_4 http://www.w3.org/2000/01/rdf-schema#label pestle http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#_5 http://www.w3.org/2000/01/rdf-schema#label extraction buffer http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#_6 http://www.w3.org/2000/01/rdf-schema#label liquid nitrogen http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#step http://purl.org/dc/elements/1.1/description Ground samples (leaf, shoot, root, and recalcitrant samples, approximately 0.5-1 g) using 1 ml of the extraction buffer with or without liquid nitrogen in mortar and pestle that are sterilized.CRITICAL STEP The procedure are carried out at room temperature except the centrifugation steps (at 4℃) as well as the time of precipitating of the nucleic acid using the isopropanol (on ice).CRITICAL STEP Severely disrupt the tissues to create the glaze mode of samples. http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#step http://purl.org/net/SMARTprotocol#hasExperimentalInput http://purl.obolibrary.org/obo/PO_0009005 http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#step http://purl.org/net/SMARTprotocol#hasExperimentalInput http://purl.obolibrary.org/obo/PO_0025034 http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#step http://purl.org/net/SMARTprotocol#hasExperimentalInput http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#_1 http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#step http://purl.org/net/SMARTprotocol#hasExperimentalInput http://www.ebi.ac.uk/efo/EFO_0000992 http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#step http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://purl.org/net/p-plan#Step http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed http://purl.obolibrary.org/obo/PO_0009005 http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed http://purl.obolibrary.org/obo/PO_0025034 http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#_2 http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed http://www.ebi.ac.uk/efo/EFO_0000992 http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#_3 http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#_4 http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#_5 http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#_6 http://www.ebi.ac.uk/efo/EFO_0000992 http://www.w3.org/2000/01/rdf-schema#label shoot http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#provenance http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#assertion http://purl.org/pav/createdWith https://nanotate.bitsfetch.com http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#assertion http://www.w3.org/ns/prov#generatedAtTime 2021-02-24T15:14:41.313929 http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#assertion http://www.w3.org/ns/prov#wasAttributedTo https://hypothes.is/users/zuluaga.lizzz http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#pubInfo http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/6x4JZnbXEeuDWO8NMPk5sQ http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/FNjJCHbYEeuipT8ixxflxQ http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/KerCfnbYEeuNBu-K6NdISQ http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/Q8mioHbYEeuTgUfhoHkUgQ http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/Z7zKcHbYEeuzMNcKBR5nMQ http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/fyGboHbYEeu2PcOWtuIVzg http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/kK_7vnbYEeu9LfdPD1KPPA http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/mci4InbXEeujlEfzlYnwTg http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/z9HP0HbXEeunbqtSwqQywQ http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#sig http://purl.org/nanopub/x/hasAlgorithm RSA http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#sig http://purl.org/nanopub/x/hasPublicKey MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#sig http://purl.org/nanopub/x/hasSignature bBIqCAPyF8g3c2Snotre9igTbITe8/nU6eYb9RgxcVWV6tZVgZ+XVIAgrlXZk4YXJtdzoo85dXXfayr5exq+ctAxHoW72jusVCL6bNnuBfcP/kro1ppLoO/ur1kzDihmwC3S9Q8HKUaGKi2d8xJuBmtNPrGtenY7MbnR/rmMAK4= http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#sig http://purl.org/nanopub/x/hasSignatureTarget http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo http://purl.org/nanopub/x/introduces http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo#step http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo http://www.w3.org/ns/prov#generatedAtTime 2021-02-24T15:14:41.313929 http://purl.org/np/RAYXMGguj-BBzZFwSCU7CwLH-LmIAkiJ3k2xDHz-UJkVo http://www.w3.org/ns/prov#wasAttributedTo https://hypothes.is/users/zuluaga.lizzz