. . . . "root" . "leaf" . "recalcitrant samples" . "recalcitrant samples" . "mortar" . "pestle" . "extraction buffer" . "liquid nitrogen" . "Ground samples (leaf, shoot, root, and recalcitrant samples, approximately 0.5-1 g) using 1 ml of the extraction buffer with or without liquid nitrogen in mortar and pestle that are sterilized.CRITICAL STEP The procedure are carried out at room temperature except the centrifugation steps (at 4℃) as well as the time of precipitating of the nucleic acid using the isopropanol (on ice).CRITICAL STEP Severely disrupt the tissues to create the glaze mode of samples." . . . . . . . . . . . . . . "shoot" . . "2021-02-24T15:14:41.313929"^^ . . . . . . . . . . . "RSA" . "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" . "bBIqCAPyF8g3c2Snotre9igTbITe8/nU6eYb9RgxcVWV6tZVgZ+XVIAgrlXZk4YXJtdzoo85dXXfayr5exq+ctAxHoW72jusVCL6bNnuBfcP/kro1ppLoO/ur1kzDihmwC3S9Q8HKUaGKi2d8xJuBmtNPrGtenY7MbnR/rmMAK4=" . . . "2021-02-24T15:14:41.313929"^^ . .