@prefix this: . @prefix sub: . @prefix bs: . @prefix np: . @prefix pav: . @prefix authority: . @prefix npx: . @prefix xsd: . @prefix rdfs: . @prefix p-plan: . @prefix prov: . @prefix sp: . @prefix dc: . sub:Head { this: np:hasAssertion sub:assertion; np:hasProvenance sub:provenance; np:hasPublicationInfo sub:pubInfo; a np:Nanopublication . } sub:assertion { rdfs:label "100mM sucrose" . rdfs:label "1% Triton -X" . rdfs:label "1% SDS" . sub:_1 rdfs:label "mosquitoes" . sub:_2 rdfs:label "mosquitoes" . sub:_3 rdfs:label "micropistle" . sub:_4 rdfs:label "Rnase A" . sub:_5 rdfs:label "Proteinase K" . sub:_6 rdfs:label "1X STE buffer " . sub:step dc:description "Grind mosquitoes in 1.5 ml eppendorf tube with micropistle in 50-100 µl 1X STE buffer ( 50mM Nacl,50mM Tris- HCL,100mM EDTA, Ph 8.0) along with 100mM sucrose. Add 1X STE buffer to a total volume of 300-500 µl for a single mosquito and 1 ml for mosquito pool like 4,6,8,10 numbers. Then add 1% SDS ,1% Triton -X , 10 µl/ ml Rnase A (20mg/ml), 20 µl/ ml Proteinase K (20mg/ml) and mix it."; sp:hasExperimentalInput sub:_1; a p-plan:Step; bs:bioSampleUsed sub:_2; bs:labEquipmentUsed sub:_3, ; bs:reagentUsed , , , sub:_4, sub:_5, sub:_6 . rdfs:label "1.5 ml eppendorf tube" . } sub:provenance { sub:assertion pav:createdWith ; prov:generatedAtTime "2021-02-24T15:57:29.331725"^^xsd:dateTime; prov:wasAttributedTo . } sub:pubInfo { sub:assertion prov:wasDerivedFrom authority:1x5l6HbfEeuNLNOMl31uWw, authority:AMqrLHbgEeu7t0tnnnh5sA, authority:JtjBvnbgEeu9UyOKVQTWOQ, authority:RU7bGnbgEeudMq9-HaiY6A, authority:V0kdJnbgEeurc_usb1nrtA, authority:aWt3LnbgEeuUGLMXogyyYg, authority:e-8U2HbfEeuds-e26QqsLg, authority:juDxOHbfEeuuK9Oh6-VO3w, authority:ql2CZHbfEeuFGXO0qGYX7g, authority:vYrwknbfEeuT7hPT-uOLOQ . sub:sig npx:hasAlgorithm "RSA"; npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB"; npx:hasSignature "aWhdnmGhJ66HdQT7ErQofYYVZALQSx0vSYV2bxZ9SyR8RnJ6zgt68Y6GIWNMMc6hLBRV5YxFXMEs4JA8KehE8EK+QlYjhEiP1q5Km6xmWcFngNWmrVfg2HQbw7jgUlZ6licoQwCN1GmXIv57ElrxNKIP3ghmVtOCEqodFcpRE8E="; npx:hasSignatureTarget this: . this: npx:introduces sub:step; prov:generatedAtTime "2021-02-24T15:57:29.331725"^^xsd:dateTime; prov:wasAttributedTo . }