@prefix this: <http://purl.org/np/RAUfXaNwZwQALTgebIITnK7ysM-tPNuQ75VFKD0SbBlu0> .
@prefix sub: <http://purl.org/np/RAUfXaNwZwQALTgebIITnK7ysM-tPNuQ75VFKD0SbBlu0#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/CHEBI_16991> rdfs:label "DNA" .
  
  <http://purl.obolibrary.org/obo/CHEBI_17824> rdfs:label " isopropanol" .
  
  <http://purl.obolibrary.org/obo/CHEBI_33697> rdfs:label "RNA" .
  
  sub:_1 rdfs:label "tubes" .
  
  sub:step dc:description "Add 700 µl of cold isopropanol to precipitate RNA or DNA and then invert tubes 3-4 times to mix the solution";
    sp:hasExperimentalOutput <http://purl.obolibrary.org/obo/CHEBI_16991>, <http://purl.obolibrary.org/obo/CHEBI_33697>;
    a p-plan:Step;
    bs:bioSampleUsed <http://purl.obolibrary.org/obo/CHEBI_16991>, <http://purl.obolibrary.org/obo/CHEBI_33697>;
    bs:labEquipmentUsed sub:_1;
    bs:reagentUsed <http://purl.obolibrary.org/obo/CHEBI_17824> .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-10T10:23:28.749259"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:P5RZ1HJJEeuWar_pp0JPJg, authority:Wj6gnoG0EeuDiLcw3qKtQA,
      authority:amrONnJJEeu0w_eniipiDg, authority:iKR-QnJJEeuOYP-b54Owxg, authority:z1ykbnJIEeu-7tMG2ROcBQ .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "eCorSCSXkCljbtieRN4QpD7krTPK2MV3lIuh6e/pnBR+LKfA9ytleKulc/debTZVXpBb9nu4s+2jGHqx1rxJbmFiSyIBqxfwReYj+bHsmhz/CuK59GgwRpQ3T3RoitqH5O2rPtbxgG+s3P4kIXzFAJSRwjdRc6+kZDObrqGj09Y=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-10T10:23:28.749259"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}