@prefix this: <http://purl.org/np/RAS8UpGgcXnoRDHf-bCkdOFShx59FpTRIyO5ODisl9UrQ> .
@prefix sub: <http://purl.org/np/RAS8UpGgcXnoRDHf-bCkdOFShx59FpTRIyO5ODisl9UrQ#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/CHEBI_16991> rdfs:label "DNA" .
  
  <http://purl.obolibrary.org/obo/CHEBI_17824> rdfs:label "isopropanol" .
  
  <http://purl.obolibrary.org/obo/CHEBI_33697> rdfs:label "RNA" .
  
  sub:_1 rdfs:label "tubes" .
  
  sub:step dc:description "Add 700 µl of cold isopropanol to precipitate RNA or DNA and then invert tubes 3-4 times to mix the solution.";
    sp:hasExperimentalOutput <http://purl.obolibrary.org/obo/CHEBI_16991>, <http://purl.obolibrary.org/obo/CHEBI_33697>;
    a p-plan:Step;
    bs:bioSampleUsed <http://purl.obolibrary.org/obo/CHEBI_16991>, <http://purl.obolibrary.org/obo/CHEBI_33697>;
    bs:labEquipmentUsed sub:_1;
    bs:reagentUsed <http://purl.obolibrary.org/obo/CHEBI_17824> .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-02-24T15:17:46.537827"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:8dG8znbZEeuMTUe2FycP_A, authority:CgWOiHbaEeugd3cNy4pUAg,
      authority:KAgs2HbaEeuTyWsCtpGVxQ, authority:QD2ETHbaEeujklcpdILvCg, authority:Xk7HtnbaEeuj6adH-EhvNw .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "TYvhNQ/K0eFKMdnUCxIvI2KTQOEXdYG/VVw5zDqDU9AFiqinBvfHf7z4Mk0gFb7qs2yc7OR7feJvHaeq2FmTZ4CL9lMirhG61hKwe5v4AcXTeMA3s4X0EWaeHVWQJmu7izDeeztXqtU2wEp7f1OYVeTjMOwyF0YzMY12RdgB62k=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-02-24T15:17:46.537827"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}