@prefix this: <http://purl.org/np/RAS8B6-t2iMp0rIQce-60YNfXbmlXovRMldA9FqbMPm_c> .
@prefix sub: <http://purl.org/np/RAS8B6-t2iMp0rIQce-60YNfXbmlXovRMldA9FqbMPm_c#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  sub:_1 rdfs:label "RNA pellets " .
  
  sub:_2 rdfs:label "RNase-free H2O" .
  
  sub:step dc:description "The RNA pellets are air-dried and dissolved in 100 µl RNase-free H2O by incubating at 65 °C for 15 min.";
    a p-plan:Step;
    bs:bioSampleUsed sub:_1;
    bs:reagentUsed sub:_2 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-11T17:35:01.489761"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:3F8vXIK5EeuaGKNhJgR3dg, authority:GL_f5mBLEeukGXPv0jvayw,
      authority:_6sf_GBKEeumT9NqxeaHDA .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "M4EN50EO6Xnow+veRnz5yNJB2QRAGfSBdLWapbJoGUwHO/vSLgUFxqzObTh2azWDjhFB9Fj676ZGQfee2WTsb+wcLgCi99VLHKKfghelTwzfSsMTA5McHP6hnglhE5XWacxMmAbFXfY5wUKF0CYVfZc99n6JGKFjX5ir0E8syj4=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-11T17:35:01.489761"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}