http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#Head http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY http://www.nanopub.org/nschema#hasAssertion http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#assertion http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY http://www.nanopub.org/nschema#hasProvenance http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#provenance http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY http://www.nanopub.org/nschema#hasPublicationInfo http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#pubInfo http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#assertion http://purl.obolibrary.org/obo/CHEBI_26710 http://www.w3.org/2000/01/rdf-schema#label 5 M NaCl http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#_1 http://www.w3.org/2000/01/rdf-schema#label supernatant http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#_2 http://www.w3.org/2000/01/rdf-schema#label chloroform: isoamyl alcohol http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#_3 http://www.w3.org/2000/01/rdf-schema#label RNAse A http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#step http://purl.org/dc/elements/1.1/description After RNAse A treatment, add one volume of chloroform: isoamyl alcohol again and mix by inversions for 5 min. The tube is centrifuged for 10 min at 5000 × g at 4°C. Transfer the clear supernatant into new 2ml tube and add half volume of 5 M NaCl to the sample and mix gently by inversions. http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#step http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://purl.org/net/p-plan#Step http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#_1 http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed http://www.bioassayontology.org/bao#BAO_0010014 http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed http://purl.obolibrary.org/obo/CHEBI_26710 http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#_2 http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#_3 http://www.bioassayontology.org/bao#BAO_0010014 http://www.w3.org/2000/01/rdf-schema#label tube http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#provenance http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#assertion http://purl.org/pav/createdWith https://nanotate.bitsfetch.com http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#assertion http://www.w3.org/ns/prov#generatedAtTime 2021-03-11T16:42:19.939476 http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#assertion http://www.w3.org/ns/prov#wasAttributedTo https://hypothes.is/users/iannotate http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#pubInfo http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/65gnrIKwEeu8ozv-JryAeQ http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/E7j6IoKxEeuOsU_XwyDh1w http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/LAccdoKxEeuiRiOOl5XFJA http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/SUBJ6IKxEeuOTeteBAH1mw http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/_rt9SIKwEeuOS7PRsXqlQA http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/nLN1sIKwEeufn8eMqZYi6g http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#sig http://purl.org/nanopub/x/hasAlgorithm RSA http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#sig http://purl.org/nanopub/x/hasPublicKey MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#sig http://purl.org/nanopub/x/hasSignature cCe5S4PTfHVNKbJDNVTTQAqxqGXwmCdNl2CL+z9K1r5knTrma1JscEMcqbOvXXiK6IXqUJ8SJ22C1RFBjxCLccSsoInb79UamARsI2c6Zh248oaxNEsSHF8BKLmWGwwDTKEZ1oqpZ7j4igSflkPVAUQ06IgSP1s6STvxRc3jNJs= http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#sig http://purl.org/nanopub/x/hasSignatureTarget http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY http://purl.org/nanopub/x/introduces http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#step http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY http://www.w3.org/ns/prov#generatedAtTime 2021-03-11T16:42:19.939476 http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY http://www.w3.org/ns/prov#wasAttributedTo https://hypothes.is/users/iannotate