. . . . "5 M NaCl" . "supernatant" . "chloroform: isoamyl alcohol" . "RNAse A" . "After RNAse A treatment, add one volume of chloroform: isoamyl alcohol again and mix by inversions for 5 min. The tube is centrifuged for 10 min at 5000 × g at 4°C. Transfer the clear supernatant into new 2ml tube and add half volume of 5 M NaCl to the sample and mix gently by inversions." . . . . . . . "tube" . . "2021-03-11T16:42:19.939476"^^ . . . . . . . . "RSA" . "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" . "cCe5S4PTfHVNKbJDNVTTQAqxqGXwmCdNl2CL+z9K1r5knTrma1JscEMcqbOvXXiK6IXqUJ8SJ22C1RFBjxCLccSsoInb79UamARsI2c6Zh248oaxNEsSHF8BKLmWGwwDTKEZ1oqpZ7j4igSflkPVAUQ06IgSP1s6STvxRc3jNJs=" . . . "2021-03-11T16:42:19.939476"^^ . .