@prefix this: <http://purl.org/np/RAORt3YgKZtkL81OJsqYkZXCoeu1VlLwnAGa4nbzeIDzU> .
@prefix sub: <http://purl.org/np/RAORt3YgKZtkL81OJsqYkZXCoeu1VlLwnAGa4nbzeIDzU#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/OBI_0000918> rdfs:label "Dulbecco's modified eagle medium" .
  
  <http://purl.obolibrary.org/obo/OBI_1000024> rdfs:label "cell pellet" .
  
  sub:_1 rdfs:label "medium" .
  
  sub:step dc:description "Discard medium and re-suspend the cell pellet in 1 ml of sterile Dulbecco's modified eagle medium.";
    sp:hasExperimentalOutput <http://purl.obolibrary.org/obo/OBI_1000024>;
    a p-plan:Step;
    bs:bioSampleUsed <http://purl.obolibrary.org/obo/OBI_1000024>;
    bs:reagentUsed <http://purl.obolibrary.org/obo/OBI_0000918>, sub:_1 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-05T10:07:43.411363"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:YFRuIn0oEeu4nkPrD6Jr7g, authority:bAQ3hH0oEeuQp9fXsMLwmQ,
      authority:h4VkJH0oEeuryj84IFo1fQ, authority:mnnxin0oEeuMqj-hee4lHA .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "bSoPMESwl5l1JiM7gvuAQm/RHiJcNZl8FG391O3HkPg99OjW0NFG8hdSF23zgDCq42TL0jdJU//wcO9m0Nwz1CCk5x6PLEh5q5hUvM63x2FDdLbzWl5miHc8Iytq++oFGcbfjNEx+VqIo61NOOWhK+GOkdgz7NY+qUY+JcJAV/k=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-05T10:07:43.411363"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}