@prefix this: <http://purl.org/np/RAOFm2cqfnhjZEZa53GKRgBBi7Koy-rq2kZZvvBA7EYoQ> .
@prefix sub: <http://purl.org/np/RAOFm2cqfnhjZEZa53GKRgBBi7Koy-rq2kZZvvBA7EYoQ#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/CHEBI_35255> rdfs:label "chloroform " .
  
  sub:_1 rdfs:label "Cell lysates" .
  
  sub:_2 rdfs:label "eppendorf tubes " .
  
  sub:step dc:description "Cell lysates were transfer into eppendorf tubes and one-fifth (volume/volume) of chloroform was added to each tube";
    a p-plan:Step;
    bs:bioSampleUsed sub:_1;
    bs:labEquipmentUsed sub:_2;
    bs:reagentUsed <http://purl.obolibrary.org/obo/CHEBI_35255> .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-11T17:36:57.258777"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:QDEDuGBJEeus9b_8w2fPjA, authority:VbBMJmBJEeu42yc92NUzfQ,
      authority:aSflmGBJEeukHrO2LDOSxA, authority:idTORmBJEeuihK-x2PObHg .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "AGiaa7EzJpwgvwa+eSHlXOmYf1CCndexhpkMcT6RgYW1rm52vEPQqkVbACpO9vFZcEJH3dpSHSJD342QcYn+F930yRKUpPRDvArzWhC8YujG/f3bWnaDWA5sUepADfWOfZZjsbIMMwyuYqVJWdRtMgt1jNUIU28V3szTihcrMvg=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-11T17:36:57.258777"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}