@prefix this: <http://purl.org/np/RAMb6wb2si75-zR2bDNpfg0lD_pWNleR9rBA4QJ64EfBo> .
@prefix sub: <http://purl.org/np/RAMb6wb2si75-zR2bDNpfg0lD_pWNleR9rBA4QJ64EfBo#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/OBI_0400155> rdfs:label "water bath" .
  
  sub:_1 rdfs:label "suspension" .
  
  sub:_2 rdfs:label "100 mg/ml lysozyme" .
  
  sub:step dc:description """Bring down temperature of suspension to ~30 °C and add 20 µl of 100 mg/ml lysozyme solution (final concentration, 2 mg/ml) to it. Incubate suspension at 37 °C for 6 h to overnight in a water bath.
Note: In case of fast-growing mycobacteria such as Mycobacterium smegmatis (M. smegmatis), go directly to step 7. However, if DNA is to be isolated from slow-growing species, follow steps 4-6 which involve lysis of cells by a physical method. Because of enhanced disruption of cells, this would significantly enhance yield.""";
    a p-plan:Step;
    bs:bioSampleUsed sub:_1;
    bs:labEquipmentUsed <http://purl.obolibrary.org/obo/OBI_0400155>;
    bs:reagentUsed sub:_2 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-10T11:18:25.462488"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:EF-yuIFSEeuet6vjBV_k6Q, authority:JfaRoIFSEeu374uFEHtqAg,
      authority:K0yIKoG8EeujNmf67OBRmw, authority:tmG8cIFREeuamj8DU21Bpw .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "AVq49EuW85NpvUlB6CB8eVMe0Jr5EYEXDCQYm9p6fF1znfEISpbUsiRzXIgCwVWYoRJ3zshHUNexjRblahiYJOvvQnwongr8MNHjhUhFhqJjgXG8trQhZ2uL0DwPB3OzOSuI9rz03wCdTzRcSrr9OSHxoqY81B5G6SBcQpeGSG8=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-10T11:18:25.462488"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}