. . . . "water bath" . "suspension" . "100 mg/ml lysozyme" . "Bring down temperature of suspension to ~30 °C and add 20 µl of 100 mg/ml lysozyme solution (final concentration, 2 mg/ml) to it. Incubate suspension at 37 °C for 6 h to overnight in a water bath.\nNote: In case of fast-growing mycobacteria such as Mycobacterium smegmatis (M. smegmatis), go directly to step 7. However, if DNA is to be isolated from slow-growing species, follow steps 4-6 which involve lysis of cells by a physical method. Because of enhanced disruption of cells, this would significantly enhance yield." . . . . . . "2021-03-10T11:18:25.462488"^^ . . . . . . "RSA" . "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" . "AVq49EuW85NpvUlB6CB8eVMe0Jr5EYEXDCQYm9p6fF1znfEISpbUsiRzXIgCwVWYoRJ3zshHUNexjRblahiYJOvvQnwongr8MNHjhUhFhqJjgXG8trQhZ2uL0DwPB3OzOSuI9rz03wCdTzRcSrr9OSHxoqY81B5G6SBcQpeGSG8=" . . . "2021-03-10T11:18:25.462488"^^ . .