@prefix this: <
http://purl.org/np/RAMb6wb2si75-zR2bDNpfg0lD_pWNleR9rBA4QJ64EfBo
> .
@prefix sub: <
http://purl.org/np/RAMb6wb2si75-zR2bDNpfg0lD_pWNleR9rBA4QJ64EfBo#
> .
@prefix bs: <
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#
> .
@prefix np: <
http://www.nanopub.org/nschema#
> .
@prefix pav: <
http://purl.org/pav/
> .
@prefix authority: <
https://hypothes.is/a/
> .
@prefix npx: <
http://purl.org/nanopub/x/
> .
@prefix xsd: <
http://www.w3.org/2001/XMLSchema#
> .
@prefix rdfs: <
http://www.w3.org/2000/01/rdf-schema#
> .
@prefix p-plan: <
http://purl.org/net/p-plan#
> .
@prefix prov: <
http://www.w3.org/ns/prov#
> .
@prefix dc: <
http://purl.org/dc/elements/1.1/
> .
sub:Head
{
this:
np:hasAssertion
sub:assertion
;
np:hasProvenance
sub:provenance
;
np:hasPublicationInfo
sub:pubInfo
;
a
np:Nanopublication
.
}
sub:assertion
{
<
http://purl.obolibrary.org/obo/OBI_0400155
>
rdfs:label
"water bath" .
sub:_1
rdfs:label
"suspension" .
sub:_2
rdfs:label
"100 mg/ml lysozyme" .
sub:step
dc:description
"""Bring down temperature of suspension to ~30 °C and add 20 µl of 100 mg/ml lysozyme solution (final concentration, 2 mg/ml) to it. Incubate suspension at 37 °C for 6 h to overnight in a water bath.
Note: In case of fast-growing mycobacteria such as Mycobacterium smegmatis (M. smegmatis), go directly to step 7. However, if DNA is to be isolated from slow-growing species, follow steps 4-6 which involve lysis of cells by a physical method. Because of enhanced disruption of cells, this would significantly enhance yield.""" ;
a
p-plan:Step
;
bs:bioSampleUsed
sub:_1
;
bs:labEquipmentUsed
<
http://purl.obolibrary.org/obo/OBI_0400155
> ;
bs:reagentUsed
sub:_2
.
}
sub:provenance
{
sub:assertion
pav:createdWith
<
https://nanotate.bitsfetch.com
> ;
prov:generatedAtTime
"2021-03-10T11:18:25.462488"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/alejandra.sandoval
> .
}
sub:pubInfo
{
sub:assertion
prov:wasDerivedFrom
authority:EF-yuIFSEeuet6vjBV_k6Q
,
authority:JfaRoIFSEeu374uFEHtqAg
,
authority:K0yIKoG8EeujNmf67OBRmw
,
authority:tmG8cIFREeuamj8DU21Bpw
.
sub:sig
npx:hasAlgorithm
"RSA" ;
npx:hasPublicKey
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
npx:hasSignature
"AVq49EuW85NpvUlB6CB8eVMe0Jr5EYEXDCQYm9p6fF1znfEISpbUsiRzXIgCwVWYoRJ3zshHUNexjRblahiYJOvvQnwongr8MNHjhUhFhqJjgXG8trQhZ2uL0DwPB3OzOSuI9rz03wCdTzRcSrr9OSHxoqY81B5G6SBcQpeGSG8=" ;
npx:hasSignatureTarget
this:
.
this:
npx:introduces
sub:step
;
prov:generatedAtTime
"2021-03-10T11:18:25.462488"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/alejandra.sandoval
> .
}