[ {
  "@graph" : [ {
    "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g",
    "@type" : [ "http://www.nanopub.org/nschema#Nanopublication" ],
    "http://www.nanopub.org/nschema#hasAssertion" : [ {
      "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#assertion"
    } ],
    "http://www.nanopub.org/nschema#hasProvenance" : [ {
      "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#provenance"
    } ],
    "http://www.nanopub.org/nschema#hasPublicationInfo" : [ {
      "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#pubInfo"
    } ]
  } ],
  "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#Head"
}, {
  "@graph" : [ {
    "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_1",
    "http://www.w3.org/2000/01/rdf-schema#label" : [ {
      "@value" : "mosquitoes"
    } ]
  }, {
    "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_10",
    "http://www.w3.org/2000/01/rdf-schema#label" : [ {
      "@value" : "1% SDS"
    } ]
  }, {
    "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_2",
    "http://www.w3.org/2000/01/rdf-schema#label" : [ {
      "@value" : "1% Triton -X"
    } ]
  }, {
    "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_3",
    "http://www.w3.org/2000/01/rdf-schema#label" : [ {
      "@value" : "mosquitoes"
    } ]
  }, {
    "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_4",
    "http://www.w3.org/2000/01/rdf-schema#label" : [ {
      "@value" : "micropistle"
    } ]
  }, {
    "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_5",
    "http://www.w3.org/2000/01/rdf-schema#label" : [ {
      "@value" : "1.5 ml eppendorf  tube"
    } ]
  }, {
    "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_6",
    "http://www.w3.org/2000/01/rdf-schema#label" : [ {
      "@value" : "1X STE buffer"
    } ]
  }, {
    "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_7",
    "http://www.w3.org/2000/01/rdf-schema#label" : [ {
      "@value" : "Proteinase K (20mg/ml)"
    } ]
  }, {
    "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_8",
    "http://www.w3.org/2000/01/rdf-schema#label" : [ {
      "@value" : "100mM sucrose"
    } ]
  }, {
    "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_9",
    "http://www.w3.org/2000/01/rdf-schema#label" : [ {
      "@value" : "Rnase A (20mg/ml)"
    } ]
  }, {
    "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#step",
    "@type" : [ "http://purl.org/net/p-plan#Step" ],
    "http://purl.org/dc/elements/1.1/description" : [ {
      "@value" : "Grind mosquitoes  in  1.5 ml eppendorf  tube with micropistle in 50-100 µl 1X STE buffer ( 50mM Nacl,50mM Tris- HCL,100mM EDTA, Ph 8.0) along with 100mM sucrose. Add 1X STE buffer to a total volume of 300-500 µl for a single mosquito and 1 ml for mosquito pool like 4,6,8,10 numbers. Then add 1% SDS ,1% Triton -X , 10 µl/ ml  Rnase A (20mg/ml), 20 µl/ ml  Proteinase K (20mg/ml) and mix it."
    } ],
    "http://purl.org/net/SMARTprotocol#hasExperimentalInput" : [ {
      "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_1"
    } ],
    "https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed" : [ {
      "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_3"
    } ],
    "https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed" : [ {
      "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_4"
    }, {
      "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_5"
    } ],
    "https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed" : [ {
      "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_10"
    }, {
      "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_2"
    }, {
      "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_6"
    }, {
      "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_7"
    }, {
      "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_8"
    }, {
      "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_9"
    } ]
  } ],
  "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#assertion"
}, {
  "@graph" : [ {
    "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#assertion",
    "http://purl.org/pav/createdWith" : [ {
      "@id" : "https://nanotate.bitsfetch.com"
    } ],
    "http://www.w3.org/ns/prov#generatedAtTime" : [ {
      "@type" : "http://www.w3.org/2001/XMLSchema#dateTime",
      "@value" : "2021-03-10T10:33:19.355370"
    } ],
    "http://www.w3.org/ns/prov#wasAttributedTo" : [ {
      "@id" : "https://hypothes.is/users/alejandra.sandoval"
    } ]
  } ],
  "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#provenance"
}, {
  "@graph" : [ {
    "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g",
    "http://purl.org/nanopub/x/introduces" : [ {
      "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#step"
    } ],
    "http://www.w3.org/ns/prov#generatedAtTime" : [ {
      "@type" : "http://www.w3.org/2001/XMLSchema#dateTime",
      "@value" : "2021-03-10T10:33:19.355370"
    } ],
    "http://www.w3.org/ns/prov#wasAttributedTo" : [ {
      "@id" : "https://hypothes.is/users/alejandra.sandoval"
    } ]
  }, {
    "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#assertion",
    "http://www.w3.org/ns/prov#wasDerivedFrom" : [ {
      "@id" : "https://hypothes.is/a/8b4bAHJNEeu01RN1eV0q-Q"
    }, {
      "@id" : "https://hypothes.is/a/ESqevnJOEeu1UX9lnqErIQ"
    }, {
      "@id" : "https://hypothes.is/a/IrIzfHJOEeujx7t3IvpXzA"
    }, {
      "@id" : "https://hypothes.is/a/OkmgznJOEeudNEPmE26k-Q"
    }, {
      "@id" : "https://hypothes.is/a/UBFH9HJOEeusy1eAko5oQw"
    }, {
      "@id" : "https://hypothes.is/a/Usa7nHJNEeuoHBdiYKYtlg"
    }, {
      "@id" : "https://hypothes.is/a/cWBYjnJMEeuOKD-DjOC3Cw"
    }, {
      "@id" : "https://hypothes.is/a/drQGkHJNEeudQGs6yWujow"
    }, {
      "@id" : "https://hypothes.is/a/lG67TnJNEeu01GMwoWiMRA"
    }, {
      "@id" : "https://hypothes.is/a/rawtGHJMEeu0J4cNJ9O11g"
    } ]
  }, {
    "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#sig",
    "http://purl.org/nanopub/x/hasAlgorithm" : [ {
      "@value" : "RSA"
    } ],
    "http://purl.org/nanopub/x/hasPublicKey" : [ {
      "@value" : "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB"
    } ],
    "http://purl.org/nanopub/x/hasSignature" : [ {
      "@value" : "Kd4Aa6fDdpMWhH77AgLNFo/GE7w50Y0bXMfwIOIVdP+2Jp7DcBZ3K28i9HOzqkwNBC9YZmAKwLlwHp818FZw1VIUbdMpPIaCSwdAsSJgsv/Ov/vuO6093NjHOignVj4QsE9+XO9WZOyFBX1XxDAbO2anaVMuoEQa4HYFefmlDOc="
    } ],
    "http://purl.org/nanopub/x/hasSignatureTarget" : [ {
      "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g"
    } ]
  } ],
  "@id" : "http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#pubInfo"
} ]