@prefix this: <http://purl.org/np/RAHvRkqwFY1KmIViYcbjIYsyN-_jC8GGHi2zIpYHQDr9E> .
@prefix sub: <http://purl.org/np/RAHvRkqwFY1KmIViYcbjIYsyN-_jC8GGHi2zIpYHQDr9E#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/CHEBI_53692> rdfs:label "histopaque" .
  
  sub:_1 rdfs:label "PBMCs" .
  
  sub:_2 rdfs:label "PBMCs" .
  
  sub:_3 rdfs:label "medium" .
  
  sub:step dc:description "Aspirate the whitish buffy coat (about 1 ml) (PBMCs) formed in the interphase between histopaque and medium.";
    sp:hasExperimentalOutput sub:_1;
    a p-plan:Step;
    bs:bioSampleUsed sub:_2;
    bs:reagentUsed <http://purl.obolibrary.org/obo/CHEBI_53692>, sub:_3 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-22T16:50:34.183995"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:1yvhHotXEeuz4DvG8rIhHQ, authority:5kJkIItXEeusjAuK0-kMpw,
      authority:9D3GvotXEeuPkDs5kwz-mA, authority:tfsnwItXEeu9HDtRSJPt9A .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "AW+/IJnd0klMjWPGPKaGXDdoBqpjMf0XW1YSTz6YJih4eHJe8BhHIPh/Do7J2KJpHohKpZYoGFaE2qwSn70J4n67HYXL+VG1WLBx6WFDTM7zJu6XtpKX9Q4y3pn9nGdF5AsjWvIpYEeA2/dPoOwuGVBxE+pKRI3reji6TEir/bs=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-22T16:50:34.183995"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}