. . . . "water bath" . "100 mg/ml lysozyme solution" . "Bring down temperature of suspension to ~30 °C and add 20 µl of 100 mg/ml lysozyme solution (final concentration, 2 mg/ml) to it. Incubate suspension at 37 °C for 6 h to overnight in a water bath.\nNote: In case of fast-growing mycobacteria such as Mycobacterium smegmatis (M. smegmatis), go directly to step 7. However, if DNA is to be isolated from slow-growing species, follow steps 4-6 which involve lysis of cells by a physical method. Because of enhanced disruption of cells, this would significantly enhance yield." . . . . . "suspension" . . "2021-03-20T05:39:47.164703"^^ . . . . . . "RSA" . "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" . "b8dX4Z3HdbViHuM7OrEfmaDCOtdttLtr0ZLCbapt3HsT2wCBkRZ65uSCufE0VO/yZt5W9VvyI/26TGvRxlN7uBve1+r+YhPi17vQ947pXt/+FAxFBzxNoKC4mbxyKjm/0hii0UxzGH62G1dt/hwSzKzG5maO9o+GasJWm+v4psI=" . . . "2021-03-20T05:39:47.164703"^^ . .