@prefix this: <http://purl.org/np/RAGrOV2KFvW4utzMtATrgT2buzdzDplSAcP4xtSVyUrSc> .
@prefix sub: <http://purl.org/np/RAGrOV2KFvW4utzMtATrgT2buzdzDplSAcP4xtSVyUrSc#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  sub:_1 rdfs:label "pellet" .
  
  sub:_2 rdfs:label "RNase free water" .
  
  sub:_3 rdfs:label "autoclaved water" .
  
  sub:step dc:description "Dissolve the pellet in 20-30 µl of RNase free water (commercial) or autoclaved water.";
    a p-plan:Step;
    bs:bioSampleUsed sub:_1;
    bs:reagentUsed sub:_2, sub:_3 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-05T11:35:43.300051"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:1_mmJn3JEeuTzZvqICVkAw, authority:7t7tkn3JEeuUtesicoitRQ,
      authority:sa-qxH3JEeuT2N-1hCdbQw, authority:xTp4gH3JEeuX7S8UqBGF_g .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "Tf9B/Zd7S20viAVYHCHo5mJF66ipxeP5OTC771b57wiYgRjgl2Dpzrp1DbBdZ6fD1xUaKXuF5RtBovI+m/VwdtaEq1O03KhMh8nOoXp7WPohaRjB0+5xuaGPF+CL4dQ5W127kncm+5KvZeUMHYQrsxWNcsrDoJVu/Y2PtwjYVGE=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-05T11:35:43.300051"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}