@prefix this: <http://purl.org/np/RAGoF9988R9n-8Q7HTyhCeiSy8sTYfOyMl8x0KMykyJ1A> .
@prefix sub: <http://purl.org/np/RAGoF9988R9n-8Q7HTyhCeiSy8sTYfOyMl8x0KMykyJ1A#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/OBI_0400115> rdfs:label "spectrophotometer" .
  
  sub:_1 rdfs:label "DNA" .
  
  sub:_2 rdfs:label "DNA" .
  
  sub:_3 rdfs:label "pellet" .
  
  sub:_4 rdfs:label "agarose gel" .
  
  sub:_5 rdfs:label "molecular biology grade water" .
  
  sub:step dc:description "Dissolve pellet in molecular biology grade water and analyze DNA by agarose gel electrophoresis and spectrophotometer for its purity and integrity";
    sp:hasExperimentalOutput sub:_1;
    a p-plan:Step;
    bs:bioSampleUsed sub:_2, sub:_3;
    bs:labEquipmentUsed <http://purl.obolibrary.org/obo/OBI_0400115>;
    bs:reagentUsed sub:_4, sub:_5 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-10T11:08:55.539168"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom <https://hypothes.is/a/-6rgXoFWEeuYIWOXjKC70A>,
      authority:5vpBkIFWEeuFOosoooc5cQ, authority:DZ9XGIFXEeu4CyOKaE8ufg, authority:I237OoFXEeuKPN9eF280hQ,
      authority:gpMSbIFXEeu6Fe_OAbvYUg, authority:wjeBMoFXEeuV1u_8Rkzwiw .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "TUr11p6UUknQ7z3ZUXmqavdWePFD10py+q62Rj39U1w6Jvu/dFeBTD/nVJRhG0TsYFulpoeKSbMbgkYeyOWqPUZ3oxLJSxR1R/gy7EtvEowAlNwidRi7kpeEV+HHn94Socgs1XyRU+txJKMaAwfrRniz2vcd+79pFmcQlgyCIb4=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-10T11:08:55.539168"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}