@prefix this: <https://protocolexchange.researchsquare.com/article/pex-1069/v1#RAFt6stRZSIiuA-w5go4rzFRQJci2Ez9yYnRw60sQMcYQ> .
@prefix sub: <https://protocolexchange.researchsquare.com/article/pex-1069/v1#RAFt6stRZSIiuA-w5go4rzFRQJci2Ez9yYnRw60sQMcYQ.> .
@prefix authority: <https://hypothes.is/a/> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix npx: <http://purl.org/nanopub/x/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
  
  sub:assertion rdfs:member sub:step .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/CHEBI_18153> rdfs:label "ethylene carbonate" .
  
  <http://purl.obolibrary.org/obo/CHEBI_29362> rdfs:label "ethylene carbonate" .
  
  <http://purl.obolibrary.org/obo/CHEBI_41609> rdfs:label "ethylene carbonate" .
  
  sub:step dc:description "DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) facilitates multiplexing in superresolution microscopy but is practically limited by slow imaging speed. We have developed DNA-PAINT-ERS, where E=ethylene carbonate, R=repeating sequence, and S=spacer, for fast and multiplexed superresolution imaging with DNA-PAINT. Here we describe detailed procedures for DNA-PAINT-ERS including reagent preparation, sample labeling, as well as image acqusition and analysis.";
    bs:bioSampleUsed "microscopy", <http://purl.obolibrary.org/obo/CHEBI_18153>, <http://purl.obolibrary.org/obo/CHEBI_29362>,
      <http://purl.obolibrary.org/obo/CHEBI_41609>;
    bs:reagentUsed <http://purl.obolibrary.org/obo/CHEBI_18153>, <http://purl.obolibrary.org/obo/CHEBI_29362>,
      <http://purl.obolibrary.org/obo/CHEBI_41609> .
}

sub:provenance {
  sub:assertion prov:generatedAtTime "2020-09-26T04:25:15.310171+00:00"^^xsd:dateTime;
    prov:wasDerivedFrom authority:Rq3gcP-wEeqPmLtbnUDy2A, authority:icECav-xEeqGzeOtUkOIRw,
      authority:ok_85v-xEeqsfZ9V8yVNxg .
}

sub:pubInfo {
  this: prov:generatedAtTime "2020-09-26T04:25:15.310171+00:00"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/a/acct:miguel.ruano@hypothes.is> .
  
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    npx:hasSignatureTarget this: .
}