@prefix this: <
https://protocolexchange.researchsquare.com/article/pex-1069/v1#RAFt6stRZSIiuA-w5go4rzFRQJci2Ez9yYnRw60sQMcYQ
> .
@prefix sub: <
https://protocolexchange.researchsquare.com/article/pex-1069/v1#RAFt6stRZSIiuA-w5go4rzFRQJci2Ez9yYnRw60sQMcYQ.
> .
@prefix authority: <
https://hypothes.is/a/
> .
@prefix bs: <
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#
> .
@prefix dc: <
http://purl.org/dc/elements/1.1/
> .
@prefix np: <
http://www.nanopub.org/nschema#
> .
@prefix prov: <
http://www.w3.org/ns/prov#
> .
@prefix rdfs: <
http://www.w3.org/2000/01/rdf-schema#
> .
@prefix xsd: <
http://www.w3.org/2001/XMLSchema#
> .
@prefix npx: <
http://purl.org/nanopub/x/
> .
sub:Head
{
this:
np:hasAssertion
sub:assertion
;
np:hasProvenance
sub:provenance
;
np:hasPublicationInfo
sub:pubInfo
;
a
np:Nanopublication
.
sub:assertion
rdfs:member
sub:step
.
}
sub:assertion
{
<
http://purl.obolibrary.org/obo/CHEBI_18153
>
rdfs:label
"ethylene carbonate" .
<
http://purl.obolibrary.org/obo/CHEBI_29362
>
rdfs:label
"ethylene carbonate" .
<
http://purl.obolibrary.org/obo/CHEBI_41609
>
rdfs:label
"ethylene carbonate" .
sub:step
dc:description
"DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) facilitates multiplexing in superresolution microscopy but is practically limited by slow imaging speed. We have developed DNA-PAINT-ERS, where E=ethylene carbonate, R=repeating sequence, and S=spacer, for fast and multiplexed superresolution imaging with DNA-PAINT. Here we describe detailed procedures for DNA-PAINT-ERS including reagent preparation, sample labeling, as well as image acqusition and analysis." ;
bs:bioSampleUsed
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http://purl.obolibrary.org/obo/CHEBI_18153
> , <
http://purl.obolibrary.org/obo/CHEBI_29362
> , <
http://purl.obolibrary.org/obo/CHEBI_41609
> ;
bs:reagentUsed
<
http://purl.obolibrary.org/obo/CHEBI_18153
> , <
http://purl.obolibrary.org/obo/CHEBI_29362
> , <
http://purl.obolibrary.org/obo/CHEBI_41609
> .
}
sub:provenance
{
sub:assertion
prov:generatedAtTime
"2020-09-26T04:25:15.310171+00:00"^^
xsd:dateTime
;
prov:wasDerivedFrom
authority:Rq3gcP-wEeqPmLtbnUDy2A
,
authority:icECav-xEeqGzeOtUkOIRw
,
authority:ok_85v-xEeqsfZ9V8yVNxg
.
}
sub:pubInfo
{
this:
prov:generatedAtTime
"2020-09-26T04:25:15.310171+00:00"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/a/acct:miguel.ruano@hypothes.is
> .
sub:sig
npx:hasAlgorithm
"RSA" ;
npx:hasPublicKey
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCAYBVNwcjF5IK+z2KRM4MqV5gG0zVrjoqZtICaimdnS2jGy8YCweNDHUD6ZWfv5p4dWQal1NAMUm1GRXJGwGYIi3ug/zL5YLU72QeDa1ziLjWa2UHHhdGzjoZy+TB87Zhe8LI4fJUXhU+jMwxCG2O613mXmmOpNQBGFJL6+VOyWwIDAQAB" ;
npx:hasSignature
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npx:hasSignatureTarget
this:
.
}