@prefix this: <http://purl.org/np/RAFGt68VzAVv0E5Mepkkl_OYi9fQ9LHGTlH2uKKA2wCog> .
@prefix sub: <http://purl.org/np/RAFGt68VzAVv0E5Mepkkl_OYi9fQ9LHGTlH2uKKA2wCog#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/CHEBI_32954> rdfs:label "3 M sodium acetate solution" .
  
  sub:_1 rdfs:label "aqueous phase" .
  
  sub:step dc:description "Add 100 µl 3 M sodium acetate solution per ml of aqueous phase obtained in step 10. Mix gently by inverting tube.";
    a p-plan:Step;
    bs:bioSampleUsed sub:_1;
    bs:labEquipmentUsed <http://www.bioassayontology.org/bao#BAO_0010014>;
    bs:reagentUsed <http://purl.obolibrary.org/obo/CHEBI_32954> .
  
  <http://www.bioassayontology.org/bao#BAO_0010014> rdfs:label "tube" .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-20T05:41:45.421654"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:16WwuIlmEeu2ocOvZA7C7Q, authority:61lbMolmEeumWbvZobofgw,
      authority:rT67solmEeu40IssMTAreQ, authority:xwNtpIlmEeunwrtoBrVj7Q .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "ZQq2Hl2HXMxund6h9AnclHlNbkmd3JfodMY/AEwkekQYlCKjgakAexqnRAhTH2ymhTelOOpYyvWRa5iD4XZF1bbvIUDrt3kBT4bV5qy9/5ARXPSZfZ3XCTCy74v8S9J78VT9Ax3wZPBp2HKY5pMkcbfbcWFZFDX71bZhuftjkDQ=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-20T05:41:45.421654"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}