@prefix this: <https://protocolexchange.researchsquare.com/article/pex-1069/v1#RAEiCKIwbKY0YHZhMFWg-DxcaWOZZSs0dC6lzDyGkKM4Y> .
@prefix sub: <https://protocolexchange.researchsquare.com/article/pex-1069/v1#RAEiCKIwbKY0YHZhMFWg-DxcaWOZZSs0dC6lzDyGkKM4Y.> .
@prefix authority: <https://hypothes.is/a/> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix npx: <http://purl.org/nanopub/x/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
  
  sub:assertion rdfs:member sub:step .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/CHEBI_29362> rdfs:label "ethylene carbonate" .
  
  <http://purl.obolibrary.org/obo/CHEBI_35225> rdfs:label "imaging buffer" .
  
  sub:step dc:description "Add imaging buffer with desired ratios of Buffer C (500 mM), ethylene carbonate, and IS-CF660R at 1-2 nM final concentration. The exact concentration of IS may need to be adjusted depending on the target and based on the imaging kinetics.";
    sp:hasExperimentalInput <http://purl.obolibrary.org/obo/CHEBI_29362>;
    bs:bioSampleUsed <http://purl.obolibrary.org/obo/CHEBI_35225>;
    bs:reagentUsed <http://purl.obolibrary.org/obo/CHEBI_35225> .
}

sub:provenance {
  sub:assertion prov:generatedAtTime "2020-09-27T00:50:41.265044+00:00"^^xsd:dateTime;
    prov:wasDerivedFrom authority:7Z1sugBbEeu9_wtvk1iAjw, authority:FrhgjABcEeu5B4dnXgvb_A,
      authority:d5JrdABbEeuatj9X3vvoXw .
}

sub:pubInfo {
  this: prov:generatedAtTime "2020-09-27T00:50:41.265044+00:00"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/a/acct:miguel.ruano@hypothes.is> .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCAYBVNwcjF5IK+z2KRM4MqV5gG0zVrjoqZtICaimdnS2jGy8YCweNDHUD6ZWfv5p4dWQal1NAMUm1GRXJGwGYIi3ug/zL5YLU72QeDa1ziLjWa2UHHhdGzjoZy+TB87Zhe8LI4fJUXhU+jMwxCG2O613mXmmOpNQBGFJL6+VOyWwIDAQAB";
    npx:hasSignature "RW2G56sxu6mjUOaOtscZ2+flr50LXuEsI+X9BcYnQ8TruLsyoDZx3UK9SwOuqoDkxyggtrGUQMhyXEWsLLvTyr1O6AM9ZVdoiEXSRgha56XTmJjgadxv1pcScOQz/Ni8G8TIm+CPiN66SILD5+JDf8tGYoB8Ksu0x2r8Gsosol8=";
    npx:hasSignatureTarget this: .
}