@prefix this: <http://purl.org/np/RAEdi_y8iLSbMtoWsgf1sBxHLPnZFDhYcBOF1e3xqonVA> .
@prefix sub: <http://purl.org/np/RAEdi_y8iLSbMtoWsgf1sBxHLPnZFDhYcBOF1e3xqonVA#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/PO_0025034> rdfs:label "leaf tissues" .
  
  sub:_1 rdfs:label "extraction buffer" .
  
  sub:step dc:description "Take one gram of frozen finely powdered leaf tissues in a new 50 mL Falcon tube and mixed with the pre-heated extraction buffer (10 ml) for one sample. Breifly, vertex for 30sec to ensure leaf material is fully mixed with buffer. Fine grind is a key to obtaining high DNA quantity with lesser artifacts of resin.";
    sp:hasExperimentalInput <http://purl.obolibrary.org/obo/PO_0025034>;
    a p-plan:Step;
    bs:bioSampleUsed <http://purl.obolibrary.org/obo/PO_0025034>;
    bs:labEquipmentUsed <http://www.bioassayontology.org/bao#BAO_0010014>;
    bs:reagentUsed sub:_1 .
  
  <http://www.bioassayontology.org/bao#BAO_0010014> rdfs:label "50 mL Falcon tube" .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-02-25T16:29:05.863279"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:5GAs8HbkEeuzrQc50uh4jA, authority:ARGpWnblEeuMGbtxQaaacw,
      authority:GBIhUnblEeuEEwtD7JL4gQ, authority:OcxTsnblEeu3W8tFpY4M7w .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "SYoPV6Z33+Kh5gmCwStU2Ck+3NZgdBE+/GUqNAS3NwTEsjoAjTHdOBENT0tzd1kiFM0v9Yn4ws47O/iuiH5XmxpzGtmSSgFrx29LDXgq/P6Dy8YM65GcQ+cn/Tbt+UesalVMFNXzEje9vfvnl1SA4YTDruknefSnBIxnyYRvR3w=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-02-25T16:29:05.863279"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}