http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#Head http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w http://www.nanopub.org/nschema#hasAssertion http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w http://www.nanopub.org/nschema#hasProvenance http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#provenance http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w http://www.nanopub.org/nschema#hasPublicationInfo http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#pubInfo http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion http://purl.obolibrary.org/obo/CHEBI_17824 http://www.w3.org/2000/01/rdf-schema#label isopropanol http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#_1 http://www.w3.org/2000/01/rdf-schema#label clear supernatant http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#_2 http://www.w3.org/2000/01/rdf-schema#label clear supernatant http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#step http://purl.org/dc/elements/1.1/description Transfer the very clear supernatant to a fresh tube , add two fold volume cold isopropanol and keep it for 1 hour at -20º celcius http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#step http://purl.org/net/SMARTprotocol#hasExperimentalInput http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#_1 http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#step http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://purl.org/net/p-plan#Step http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#_2 http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed http://www.bioassayontology.org/bao#BAO_0010014 http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed http://purl.obolibrary.org/obo/CHEBI_17824 http://www.bioassayontology.org/bao#BAO_0010014 http://www.w3.org/2000/01/rdf-schema#label tube http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#provenance http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion http://purl.org/pav/createdWith https://nanotate.bitsfetch.com http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion http://www.w3.org/ns/prov#generatedAtTime 2021-03-10T10:40:34.285030 http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion http://www.w3.org/ns/prov#wasAttributedTo https://hypothes.is/users/alejandra.sandoval http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#pubInfo http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/1AI99nJPEeuV0jNgsqleMQ http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/3_u-coG2EeuImTcXZHRdwA http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/FPj_NHJQEeusgDdsyq658w http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/Lh_nmHJQEeuT8WfgFIk0fw http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#sig http://purl.org/nanopub/x/hasAlgorithm RSA http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#sig http://purl.org/nanopub/x/hasPublicKey MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#sig http://purl.org/nanopub/x/hasSignature U1qf30RAXfxn1/xwTczDFhzx1wBXAs8h4UBAJ4OiTXKiqKhZXKRqbrholoeYD6OmZ8aEf4Z8QLIMPo2jO/5HtS6Sb81GV/WhsNeoMxYp7eJsEDJiVIeR2Qz8dq3xJnwLVwt6IK6cj57brAhPPZCYiv5YUR8oJ4DkZKpv4zV+YVk= http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#sig http://purl.org/nanopub/x/hasSignatureTarget http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w http://purl.org/nanopub/x/introduces http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#step http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w http://www.w3.org/ns/prov#generatedAtTime 2021-03-10T10:40:34.285030 http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w http://www.w3.org/ns/prov#wasAttributedTo https://hypothes.is/users/alejandra.sandoval