@prefix this: <http://purl.org/np/RA8vn2YWrFMiWvNBVO2inHF2gTHdUjQwkF8PVz4txJLyM> .
@prefix sub: <http://purl.org/np/RA8vn2YWrFMiWvNBVO2inHF2gTHdUjQwkF8PVz4txJLyM#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/OBI_0000079> rdfs:label "culture medium" .
  
  <http://purl.obolibrary.org/obo/OBI_0002488> rdfs:label "pipette" .
  
  sub:_1 rdfs:label "mycobacteria" .
  
  sub:_2 rdfs:label "mycobacteria" .
  
  sub:_3 rdfs:label "TE" .
  
  sub:step dc:description """Harvest mycobacteria by centrifuging 20-40 ml log phase culture (O.D.600 ≈ 0.8) at 2,000 x g for 10 min, room temperature. Carefully discard culture medium and add 10 ml TE (at room temperature) buffer to pellet. Mix gently with a pipette.
Note: If isolating DNA from pathogenic mycobacteria such as M. tuberculosis, step 1 and 2 must be performed in a BSL-3 facility.""";
    sp:hasExperimentalInput sub:_1;
    a p-plan:Step;
    bs:bioSampleUsed sub:_2;
    bs:labEquipmentUsed <http://purl.obolibrary.org/obo/OBI_0002488>;
    bs:reagentUsed <http://purl.obolibrary.org/obo/OBI_0000079>, sub:_3 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-20T05:40:20.117251"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:dQ479ojyEeuhgEtOK1bBSQ, authority:hhu0tIjyEeusPSvAFdQnZA,
      authority:oIhcJojyEeufutcrzCqdww, authority:tGMHbojyEeuY1HvFKqp2_Q, authority:yYC5_IjyEeumSD_TQuFGDQ .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "IrbrgIvAYI8Z+G3fhf1EJePUEr7e60jZ4xDhRSU+gEjhuBrD8VSo5jwSfYEqlxRizLBQisYUbABXKb6lq9BjaeCfe6F8UJJdkzAyO/Y4Py5yu9uBF7OgwA5Div7EMMW45CEs2UncygnZk/oXiFTqx8OAdpi5Rj2rJTVnMl5248Q=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-20T05:40:20.117251"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}